Pseudodesulfovibrio nedwellii sp. nov., a mesophilic sulphate-reducing bacterium isolated from a xenic culture of an anaerobic heterolobosean protist
Pseudodesulfovibrio nedwellii sp. nov., a mesophilic sulphate-reducing bacterium isolated from a xenic culture of an anaerobic heterolobosean protist
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DOI：
10.1099/ijsem.0.005826
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发表时间：
2023-01-01
影响因子：
2.8
通讯作者：
Kondo，Ryuji
中科院分区：
文献类型：
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作者：
Kondo，Ryuji
从日本福井盐湖日流湖的硫化沉积物中获得的厌氧原生生物异种培养物中分离出一种新型硫酸盐还原细菌 SYKT 菌株。基于16S rRNA基因序列的系统发育分析结果表明，SYKT与假脱硫弧菌属成员聚为一簇。菌株SYKT的近亲是Pseudodesulfovibrio sediminisSF6T，16S rRNA基因序列同一性达97.43 %。 SYKT 与假脱硫弧菌属物种之间的数字 DNA-DNA 杂交和平均核苷酸同一性值均低于物种划分的相应阈值，表明 SYKT 代表假脱硫弧菌属的新物种。细胞大小为 1.7–3.7×0.2–0.5μm，革兰氏染色阴性，专性厌氧，通过单极鞭毛运动，呈弯曲杆状或乙状结肠形状。在pH 6.0至9.5（最佳pH 8.0-9.0）的盐水条件和10-30℃（最佳25℃）的温度下观察细胞生长。 SYKT 使用乳酸、丙酮酸、富马酸、甲酸和 H2 作为电子供体。它使用硫酸盐、亚硫酸盐、硫代硫酸盐和硫作为末端电子受体。丙酮酸和富马酸被发酵。主要细胞脂肪酸为anteiso-C15 ： 0、C16 ： 0、anteiso-C17 ： 1ω9c、求和特征3（C16 ： 1ω6cand/或C16 ： 1ω7c）和求和特征8（C18 ： 1ω7cand/或） C18 : 1ω6c)。 SYKT的DNA G+C含量为49.4 mol%。根据遗传和表型特征，SYKT 被确定为假脱硫弧菌属的一个新种，命名为假脱硫弧菌 nedwelliisp。十一月建议采用 SYKT 型菌株（=DSM 114958T=JCM 35746T）。
A novel sulphate-reducing bacterium, strain SYKT, was isolated from a xenic culture of an anaerobic protist obtained from a sulphidogenic sediment of the saline Lake Hiruga in Fukui, Japan. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that SYKTclustered with the members of the genusPseudodesulfovibrio. The closest relative of strain SYKTwasPseudodesulfovibrio sediminisSF6T, with 16S rRNA gene sequence identity of 97.43 %. Digital DNA–DNA hybridisation and average nucleotide identity values between SYKTand species of the genusPseudodesulfovibriofell below the respective thresholds for species delineation, indicating that SYKTrepresents a novel species of the genusPseudodesulfovibrio. Cells measured 1.7–3.7×0.2–0.5 µm in size and were Gram-stain-negative, obligately anaerobic, motile by means of a single polar flagellum and had a curved rod or sigmoid shape. Cell growth was observed under saline conditions from pH 6.0 to 9.5 (optimum pH 8.0–9.0) and at a temperature of 10–30 °C (optimum 25 °C). SYKTused lactate, pyruvate, fumarate, formate and H2as electron donors. It used sulphate, sulphite, thiosulphate and sulphur as terminal electron acceptors. Pyruvate and fumarate were fermented. Major cellular fatty acids were anteiso-C15 : 0, C16 : 0, anteiso-C17 : 1ω9c, summed feature 3 (C16 : 1ω6cand/or C16 : 1ω7c) and summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c). The DNA G+C content of SYKTwas 49.4 mol%. On the basis of the the genetic and phenotypic features, SYKTwas determined to represent a novel species of the genusPseudodesulfovibriofor which the namePseudodesulfovibrio nedwelliisp. nov. is proposed with type strain SYKT(=DSM 114958T=JCM 35746T).