Development of microsatellite markers in a clonal perennial herb, Convallaria keiskei

Development of microsatellite markers in a clonal perennial herb, Convallaria keiskei
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DOI：
10.1111/j.1471-8286.2006.01465.x
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发表时间：
2006-12
期刊：
Molecular Ecology Notes
影响因子：
--
通讯作者：
Kiwako S. Araki;C. Lian;K. Shimatani;M. Ohara
Kiwako S. Araki;C. Lian;K. Shimatani;M. Ohara
中科院分区：
其他
文献类型：
--
作者：
Kiwako S. Araki;C. Lian;K. Shimatani;M. Ohara

文献摘要

相似文献

利用双抑制聚合酶链反应（PCR）技术和改进技术，从多年生草本植物康华（Convallaria keiskei）基因组DNA中获得8个多态微卫星简单序列重复（SSR）位点。这些标记每个位点有4至10个等位基因，从日本北海道一个种群采集的82份样本中鉴定出29种基因型。观察到的杂合度为0.241 ~ 0.862，期望杂合度为0.639 ~ 0.825。这些SSR标记可用于油菜的基因鉴定和遗传多样性评价。
We developed eight polymorphic microsatellite simple sequence repeat (SSR) loci from genomic DNA of a clonal perennial herb, Convallaria keiskei, using a dual-suppression-polymerase chain reaction (PCR) technique and an improved technique. These markers with four to 10 alleles per locus identified 29 genotypes in 82 samples collected from a population in Hokkaido, Japan. The observed and expected heterozygosities ranged from 0.241 to 0.862 and from 0.639 to 0.825, respectively. These SSR markers will be available to identify genets and evaluate genetic diversity of C. keiskei.