Serial crystallography using automated drop dispensing.
Serial crystallography using automated drop dispensing.
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DOI：
10.1107/s1600577521006160
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发表时间：
2021-09-01
影响因子：
2.5
通讯作者：
DePonte D
中科院分区：
文献类型：
--
作者：
Su Z;Cantlon J;Douthit L;Wiedorn M;Boutet S;Kern J;Yoon CH;DePonte D
描述了自动样品交付和按需投放方法的开发。自动化脉冲液相样品传输有可能大大提高脉冲 X 射线设施中样品和光子的使用效率。在这项工作中，演示了一种自动按需滴注 (DOD) 系统，该系统可加速串行飞秒晶体学 (SFX) 的样品交换。测试了四种不同的蛋白质晶体浆液，并且通过自动样品循环系统进一步改进了该技术，其有效性通过索引结果得到验证。在这里，高通量 SFX 筛选被证明可以在自由电子激光设施上进行，交叉污染的风险非常低，停机时间也很短。该技术的发展将显着减少样品消耗，并能够对难以大量结晶的蛋白质进行结构测定。这项工作也为自动化样品交付奠定了基础。
The development of automated sample delivery and drop on demand methods is described. Automated, pulsed liquid-phase sample delivery has the potential to greatly improve the efficiency of both sample and photon use at pulsed X-ray facilities. In this work, an automated drop on demand (DOD) system that accelerates sample exchange for serial femtosecond crystallography (SFX) is demonstrated. Four different protein crystal slurries were tested, and this technique is further improved here with an automatic sample-cycling system whose effectiveness was verified by the indexing results. Here, high-throughput SFX screening is shown to be possible at free-electron laser facilities with very low risk of cross contamination and minimal downtime. The development of this technique will significantly reduce sample consumption and enable structure determination of proteins that are difficult to crystallize in large quantities. This work also lays the foundation for automating sample delivery.