Activation of an Endogenous Retrotransposon Associated with Epigenetic Changes in Lotus japonicus: A Tool for Functional Genomics in Legumes
Activation of an Endogenous Retrotransposon Associated with Epigenetic Changes in Lotus japonicus: A Tool for Functional Genomics in Legumes
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DOI：
10.3835/plantgenome2013.04.0009
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发表时间：
2013-11-01
期刊：
影响因子：
4.2
通讯作者：
Hayashi, Makoto
中科院分区：
文献类型：
--
作者：
Fukai, Eigo;Stougaard, Jens;Hayashi, Makoto
长末端重复反转录转座子在显花植物基因组中占有很大比例。尽管数量众多，但大多数人都保持沉默，很少换位。高活性反转录转座子的一个例子是模式豆科植物Lotus Rupel（Regel）K的Lotus反转录转座子1（LORE 1）。Larsen（syn.百脉根变种Regel）。LORE 1有几个不寻常的特征，这对于研究进化基因组学和将LORE 1用作遗传工具都很有趣。本文就LORE 1基因的特性及其作为吉普赛超家族中一个含染色体结构域的分支的生物学意义进行综述。然后我们讨论了使用内源转座因子作为诱变剂在植物中产生基因标签群体的可能性和方法。
Long terminal repeat retrotransposons occupy a large portion of genomes in flowering plants. In spite of their abundance, the majority are silenced and rarely transpose. One of the examples of a highly active retrotransposon is Lotus Retrotransposon 1 (LORE1), of the model legume Lotus japonicus (Regel) K. Larsen (syn. Lotus corniculatus L. var. japonicus Regel). LORE1 has several unusual characteristics that are interesting both for studying evolutional genomics and for the use of LORE1 as a genetic tool. In this review, we present the characteristics of LORE1 and discuss the biological significance of LORE1 as a member of chromovirus, a chromodomain containing clade of the Gypsy superfamily. Then we discuss possibilities and methodologies for using endogenous transposable elements as mutagens to generate gene tagging populations in plants.