Spectral Karyotyping
Spectral Karyotyping
复制标题
DOI：
10.1017/s1431927600007923
复制
发表时间：
1997-08
影响因子：
2.8
通讯作者：
T. Ried;M. Macville;S. Manoir;T. Veldman;Z. Weaver;M. Liyanage;E. Schröck
中科院分区：
文献类型：
--
作者：
T. Ried;M. Macville;S. Manoir;T. Veldman;Z. Weaver;M. Liyanage;E. Schröck
在人类白血病和实体瘤中，细胞遗传学分析提供了具有诊断和预后重要性的关键信息。然而，通常仅使用传统的染色体显带技术很难明确地表征所有异常染色体。我们开发了一种称为光谱核型分析 (SKY) 的新方法，该方法基于 24 个差异标记的人类染色体涂色探针的杂交，可同时对所有人类染色体进行颜色识别。这种基因组扫描方法结合了傅里叶光谱、CCD 成像和光学显微镜，以可视化差异标记的染色体涂色探针的杂交。最重要的分析算法之一是基于光谱的分类算法，该算法能够识别图像中的多个不同光谱并以分类颜色突出显示。这允许根据所有人类染色体的光谱为它们分配特定的分类颜色。该算法假设每条染色体的（参考）光谱已被测量并存储在计算机的参考库中
In human leukemia and solid tumors cytogenetic analysis provides critical information of diagnostic and prognostic importance. Frequently however, the unambiguous characterization of all aberrant chromosomes is difficult using conventional chromosome banding techniques alone. We have developed a novel approach, termed spectral karyotyping (SKY), based on the hybridization of 24 differentially labeled human chromosome painting probes that allows the simultaneous color identification of all human chromosomes. This genome scanning method combines Fourier spectroscopy, CCD-imaging, and optical microscopy to visualize the hybridization of differentially labeled chromosomes painting probes. One of the most important analysis algorithms is the spectral-based classification algorithm that enables multiple different spectra in the image to be identified and highlighted in classification-colors. This allows assignment of a specific classification-color to all human chromosomes based on their spectra. This algorithm assumes that the (reference) spectrum of each chromosome has been measured and stored in a reference library in the computer