CALCIUM-DEPENDENT HEPARIN-LIKE LIGANDS FOR L-SELECTIN IN NONLYMPHOID ENDOTHELIAL-CELLS
CALCIUM-DEPENDENT HEPARIN-LIKE LIGANDS FOR L-SELECTIN IN NONLYMPHOID ENDOTHELIAL-CELLS
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DOI：
10.1126/science.7687382
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发表时间：
1993-07-23
期刊：
影响因子：
56.9
通讯作者：
VARKI, A
中科院分区：
文献类型：
--
作者：
NORGARDSUMNICHT, KE;VARKI, NM;VARKI, A
L-选择素是一种钙依赖性哺乳动物凝集素，通过识别淋巴结中高内皮微静脉上的唾液酸化配体介导淋巴细胞运输。虽然L-选择素可能介导中性粒细胞外渗到非淋巴组织，没有相应的配体已被确定。用L-选择素嵌合体（LS-Rg）染色培养的内皮细胞显示配体的内部池。硫-35-标记的硫酸盐的代谢标记显示肝素裂解酶敏感的配体，结合LS-Rg在钙依赖性，唾液酸独立的方式。一部分市售肝素与LS-Rg结合，LS-Rg与肝素-琼脂糖结合，均以钙依赖性方式。因此，L-选择素识别内皮肝素样链，其可能是介导白细胞运输的生理配体。
L-Selectin is a calcium-dependent mammalian lectin that mediates lymphocyte trafficking by recognizing sialylated ligands on high endothelial venules in lymph nodes. Although L-selectin probably mediates neutrophil extravasation into nonlymphoid tissues, no corresponding ligand has been characterized. Staining of cultured endothelial cells with an L-selectin chimera (LS-Rg) showed an internal pool of ligands. Metabolic labeling with sulfur-35-labeled sulfate revealed heparin lyase-sensitive ligands that bound LS-Rg in a calcium-dependent, sialic acid-independent manner. A fraction of commercial heparin bound to LS-Rg and LS-Rg bound to heparin-agarose, both in a calcium-dependent manner. Thus, L-selectin recognizes endothelial heparin-like chains, which could be physiological ligands mediating leucocyte trafficking.