Extending the loop design for two-channel microarray experiments
Extending the loop design for two-channel microarray experiments
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DOI：
10.1017/s0016672307008476
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发表时间：
2006-12-01
期刊：
影响因子：
--
通讯作者：
Hua, Jun
中科院分区：
文献类型：
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作者：
Altman, Naomi S.;Hua, Jun
Kerr和Churchill的环路设计是将大小为2的不完整块巧妙地应用于双通道微阵列实验。在本文中，我们扩展了环路设计，包括更多的重复，生物和技术复制，多因素实验和阻断。对于任何给定数量的阵列，循环和扩展循环设计都比参考设计更有效。我们还表明，在环路设计中添加新的处理需要与在参考设计中添加处理相同数量的额外阵列，并且功率增益更大。考虑到扩展环路设计的灵活性及其功率，我们建议这些应该是大多数使用双通道微阵列实验的选择设计。
The loop design of Kerr and Churchill is a clever application of incomplete blocks of size 2 to two-channel microarray experiments. In this paper, we extend the loop design to include more replicates, biological and technical replication, multi-factor experiments, and blocking. Loop and extended loop designs are shown to be more efficient than the reference design for any given number of arrays. We also show that adding new treatments to a loop design requires the same number of additional arrays as adding treatments to a reference design, with a greater gain in power. Given the flexibility of extended loop designs and their power, we propose that these should be the designs of choice for most experiments using two-channel microarrays.