Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures.
Extracellular vimentin is sufficient to promote cell attachment, spreading, and motility by a mechanism involving N-acetyl glucosamine-containing structures.
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DOI：
10.1016/j.jbc.2023.104963
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发表时间：
2023-08
影响因子：
4.8
通讯作者：
Janmey, Paul A.
中科院分区：
文献类型：
--
作者：
Bucki, Robert;Iwamoto, Daniel, V;Shi, Xuechen;Kerr, Katherine E.;Byfield, Fitzroy J.;Suprewicz, Lukasz;Sklodowski, Karol;Sutaria, Julian;Misiak, Pawe l;Wilczewska, Agnieszka Z.;Ramachandran, Sekar;Wolfe, Aaron;Thanh, Minh-Tri Ho;Whalen, Eli;Patteson, Alison E.;Janmey, Paul A.
波形蛋白中间丝是间充质细胞骨架的一部分，但在通常与炎症相关的病理条件下，波形蛋白丝由于磷酸化或瓜氨酸化而解聚，波形蛋白低聚物被分泌或释放到细胞外环境中。在细胞外空间，vimentin可以结合细胞表面和细胞外基质，细胞外vimentin与细胞之间的相互作用可以触发细胞功能的变化，如成纤维细胞活化成纤维化表型。细胞外波形蛋白结合外细胞膜的机制以及波形蛋白是否可以单独作为细胞的粘附锚在很大程度上是未知的。在这里，我们展示了各种细胞类型（正常和无波形蛋白的成纤维细胞、间充质干细胞和A549肺癌细胞）附着在与波形蛋白共价连接的聚丙烯酰胺水凝胶底物上并在其上扩散。利用牵引力显微镜和球体膨胀试验，我们描述了不同细胞类型对细胞外波形蛋白的反应。透明质酸降解酶、透明质酸合成酶抑制剂、可溶性肝素或n-乙酰氨基葡萄糖可以抑制细胞附着在蛋白包被表面上并在其上扩散，所有这些处理对与胶原包被水凝胶结合的相同细胞类型几乎没有影响。这些研究强调了底物结合的波形蛋白作为细胞配体的有效性，并表明碳水化合物结构，包括糖萼和含有n -乙酰氨基葡萄糖的糖基化细胞表面蛋白，形成了一类新的细胞外波形蛋白粘附受体，可以直接支持细胞粘附到底物或微调糖萼粘附特性。
Vimentin intermediate filaments form part of the cytoskeleton of mesenchymal cells, but under pathological conditions often associated with inflammation, vimentin filaments depolymerize as the result of phosphorylation or citrullination, and vimentin oligomers are secreted or released into the extracellular environment. In the extracellular space, vimentin can bind surfaces of cells and the extracellular matrix, and the interaction between extracellular vimentin and cells can trigger changes in cellular functions, such as activation of fibroblasts to a fibrotic phenotype. The mechanism by which extracellular vimentin binds external cell membranes and whether vimentin alone can act as an adhesive anchor for cells is largely uncharacterized. Here, we show that various cell types (normal and vimentin null fibroblasts, mesenchymal stem cells, and A549 lung carcinoma cells) attach to and spread on polyacrylamide hydrogel substrates covalently linked to vimentin. Using traction force microscopy and spheroid expansion assays, we characterize how different cell types respond to extracellular vimentin. Cell attachment to and spreading on vimentin-coated surfaces is inhibited by hyaluronic acid degrading enzymes, hyaluronic acid synthase inhibitors, soluble heparin or N-acetyl glucosamine, all of which are treatments that have little or no effect on the same cell types binding to collagen-coated hydrogels. These studies highlight the effectiveness of substrate-bound vimentin as a ligand for cells and suggest that carbohydrate structures, including the glycocalyx and glycosylated cell surface proteins that contain N-acetyl glucosamine, form a novel class of adhesion receptors for extracellular vimentin that can either directly support cell adhesion to a substrate or fine-tune the glycocalyx adhesive properties.
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影响因子：
3.4
作者：
Basta MD;Paulson H;Walker JL
通讯作者：
Walker JL
将多个水平的流体剪应力应用于聚丙烯酰胺凝胶上的内皮细胞。
影响因子：
6.1
作者：
Galie PA;van Oosten A;Chen CS;Janmey PA
通讯作者：
Janmey PA
影响因子：
5.5
作者：
Menko AS;Romisher A;Walker JL
通讯作者：
Walker JL
影响因子：
4.3
作者：
Ise, Hirohiko;Kobayashi, Satoshi;Akaike, Toshihiro
通讯作者：
Akaike, Toshihiro
DOI：
10.1073/pnas.2113874119
发表时间：
2022-02-08
影响因子：
11.1
作者：
Amraei R;Xia C;Olejnik J;White MR;Napoleon MA;Lotfollahzadeh S;Hauser BM;Schmidt AG;Chitalia V;Mühlberger E;Costello CE;Rahimi N
通讯作者：
Rahimi N