Severe acute respiratory syndrome coronavirus Orf3a protein interacts with caveolin
Severe acute respiratory syndrome coronavirus Orf3a protein interacts with caveolin
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DOI：
10.1099/vir.0.82856-0
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Jameel, Shahid
中科院分区：
文献类型：
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作者：
Padhan, Kartika;Tamar, Charu;Jameel, Shahid
orf 3a（也称为X1或U274）基因是严重急性呼吸综合征冠状病毒基因组中最大的独特开放阅读框架，并已被提出编码具有三个跨膜结构域和一个大的胞质结构域的蛋白质。最近的研究表明，3a蛋白可能在病毒生命周期中发挥结构性作用，尽管其机制尚未确定。在此，显示了3a蛋白在各种体外系统中的表达，其已定位于高尔基体区域，并且其在转染细胞中的膜拓扑结构已得到证实。据报道，在3a蛋白中存在三个潜在的小窝蛋白-1结合位点。通过使用各种生物化学、生物物理学和遗传学技术，证明了3a蛋白与小窝蛋白-1的相互作用。3a中的任何一个潜在地点。蛋白质足以进行这种相互作用。这些结果进行了讨论相对于3A蛋白在病毒的生命周期中的可能作用。
The orf3a (also called X1 or U274) gene is the largest unique open reading frame in the severe acute respiratory syndrome coronavinus genome and has been proposed to encode a protein with three transmembrane domains and a large cytoplasmic domain. Recent work has suggested that the 3a protein may play a structural role in the viral life cycle, although the mechanisms for this remain uncharacterized. Here, the expression of the 3a protein in various in vitro systems is shown, it has been localized to the Golgi region and its membrane topology in transfected cells has been confirmed. Three potential caveolin-1-binding sites were reported to be present in the 3a protein. By using various biochemical, biophysical and genetic techniques, interaction of the 3a protein with caveolin-1 is demonstrated. Any one of the potential sites in the 3a. protein was sufficient for this interaction. These results are discussed with respect to the possible roles of the 3a protein in the viral life cycle.