Manganese-enhanced T1 mapping to quantify myocardial viability: validation with 18F-fluorodeoxyglucose positron emission tomography
Manganese-enhanced T1 mapping to quantify myocardial viability: validation with 18F-fluorodeoxyglucose positron emission tomography
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DOI：
10.1038/s41598-020-58716-x
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发表时间：
2020-02-06
影响因子：
4.6
通讯作者：
Semple, Scott I.
中科院分区：
文献类型：
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作者：
Spath, Nick;Tavares, Adriana;Semple, Scott I.
钆螯合物在心血管磁共振成像（MRI）中作为被动血管内和细胞外空间标记物被广泛应用。锰，一种具有生物活性的顺磁性钙类似物，提供了新的细胞内心肌组织特征。我们之前的研究表明，锰增强MRI （MEMRI）比钆延迟增强MRI （DEMRI）更准确地量化心肌梗死。在这里，我们将MEMRI与金标准的f -18-氟脱氧葡萄糖（F-18-FDG）正电子发射断层扫描（PET）存活率进行了比较，评估了MEMRI评估心肌活力的潜力。在10-12周对雄性Sprague-Dawley大鼠（n=13）进行冠状动脉结扎手术，并进行双MEMRI和F-18-FDG PET成像。MEMRI是用未螯合的（EVP1001-1）或螯合的（mangafodipir）锰来实现的。T-1定位MRI后进行F-18-FDG微pet，取组织进行组织学对比。MEMRI和PET显示与组织学吻合良好，但原生T-1低估了梗死面积。与锰剂无关，MEMRI、PET和MTC的定量结果相似。MEMRI与PET的一致性优于原生T-1。MEMRI显示出与PET和MTC极好的一致性。心肌MEMRI T-1与F-18-FDG标准摄取值和内流常数相关，但与原生T-1无关。我们的研究结果表明，MEMRI识别和量化心肌活力，在心肌疾病和再生治疗中具有重要的临床应用潜力。
Gadolinium chelates are widely used in cardiovascular magnetic resonance imaging (MRI) as passive intravascular and extracellular space markers. Manganese, a biologically active paramagnetic calcium analogue, provides novel intracellular myocardial tissue characterisation. We previously showed manganese-enhanced MRI (MEMRI) more accurately quantifies myocardial infarction than gadolinium delayed-enhancement MRI (DEMRI). Here, we evaluated the potential of MEMRI to assess myocardial viability compared to gold-standard F-18-fluorodeoxyglucose (F-18-FDG) positron emission tomography (PET) viability. Coronary artery ligation surgery was performed in male Sprague-Dawley rats (n=13) followed by dual MEMRI and F-18-FDG PET imaging at 10-12 weeks. MEMRI was achieved with unchelated (EVP1001-1) or chelated (mangafodipir) manganese. T-1 mapping MRI was followed by F-18-FDG micro-PET, with tissue taken for histological correlation. MEMRI and PET demonstrated good agreement with histology but native T-1 underestimated infarct size. Quantification of viability by MEMRI, PET and MTC were similar, irrespective of manganese agent. MEMRI showed superior agreement with PET than native T-1. MEMRI showed excellent agreement with PET and MTC viability. Myocardial MEMRI T-1 correlated with F-18-FDG standard uptake values and influx constant but not native T-1. Our findings indicate that MEMRI identifies and quantifies myocardial viability and has major potential for clinical application in myocardial disease and regenerative therapies.