GLUTAMINE PRESERVES LIVER GLUTATHIONE AFTER LETHAL HEPATIC-INJURY
GLUTAMINE PRESERVES LIVER GLUTATHIONE AFTER LETHAL HEPATIC-INJURY
复制标题
DOI：
10.1097/00000658-199202000-00004
复制
发表时间：
1992-02-01
影响因子：
9
通讯作者：
WILMORE, DW
中科院分区：
文献类型：
--
作者：
HONG, RW;ROUNDS, JD;WILMORE, DW
谷胱甘肽（GSH）是一种主要的抗氧化剂，可保护组织免受自由基损伤。谷氨酰胺增强宿主防御，可能在GSH合成中起重要作用。对乙酰氨基酚毒性导致肝GSH耗竭和肝坏死。作者假设，谷氨酰胺补充营养可以增加肝脏GSH储存，减少对乙酰氨基酚过量后的肝损伤和死亡。Wistar大鼠接受标准全胃肠外营养（TPN）溶液（STD）或等热量等氮谷氨酰胺补充溶液（GLN）。在喂食的第5天，给予动物对乙酰氨基酚（400 mg/kg，腹腔注射），然后在不同的时间点处死。标准TPN溶液动物的肝脏谷胱甘肽迅速耗尽，而GLN动物对这种下降具有抵抗力，并迅速补充肝脏GSH储存。与STD动物相比，补充谷氨酰胺的动物维持较高的血浆谷氨酰胺浓度，肝酶升高较少，并发症明显较少。作者得出结论，补充谷氨酰胺的营养可以保护肝脏谷胱甘肽，保护肝脏，并提高对乙酰氨基酚中毒期间的存活率。谷氨酰胺可以通过增强抗氧化保护来增强宿主防御。
Glutathione (GSH) is a major antioxidant that protects tissues from free radical injury. Glutamine augments host defenses and may be important in GSH synthesis. Acetaminophen toxicity causes hepatic GSH depletion and hepatic necrosis. The authors hypothesized that glutamine-supplemented nutrition would enhance liver GSH stores and diminish hepatic injury and death after acetaminophen overdose. Wistar rats received either a standard total parenteral nutrition (TPN) solution (STD) or an isocaloric, isonitrogenous glutamine-supplemented solution (GLN). On the 5th day of feeding, animals were given acetaminophen (400 mg/kg intraperitoneally) and then killed at various time points. Standard TPN solution animals had a rapid depletion of hepatic glutathione, whereas GLN animals were resistant to this drop and rapidly repleted hepatic GSH stores. Glutamine-supplemented animals maintained higher plasma glutamine concentrations, had lesser elevations in hepatic enzymes, and sustained significantly fewer complications compared with STD animals. The authors conclude that glutamine-supplemented nutrition preserves hepatic glutathione, protects the liver, and improves survival during acetaminophen toxicity. Glutamine may augment host defenses by enhancing antioxidant protection.