Expression and distribution of JNK/SAPK-associated scaffold protein JSAP1 in developing and adult mouse brain
Expression and distribution of JNK/SAPK-associated scaffold protein JSAP1 in developing and adult mouse brain
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DOI：
10.1111/j.1471-4159.2006.03835.x
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发表时间：
2006-06-01
影响因子：
4.7
通讯作者：
Watanabe, Masahiko
中科院分区：
文献类型：
--
作者：
Miura, Eriko;Fukaya, Masahiro;Watanabe, Masahiko
c-Jun N-末端激酶（JNK）是三种主要的丝裂原活化蛋白激酶（MAPK）之一，在细胞内外刺激的各种细胞过程中起关键作用。JNK/SAPK相关蛋白1（JSAP 1，也称为JIP 3）是一种JNK相关支架，其控制JNK信号级联的特异性和效率。在这里，我们研究了它在小鼠大脑中的表达。JSAP 1 mRNA在发育和成年大脑中表达，显示出与JNK 1 -3 mRNA相似的空间模式。在胚胎中，JSAP 1免疫标记是强烈的祖细胞在整个大脑的脑室区和小脑的外部颗粒层，并为神经元和神经胶质细胞分化的外套带。在成年人中，JSAP 1分布在各种神经元和Bergmann胶质细胞中，在纹状体胆碱能中间神经元、端脑小白蛋白阳性中间神经元和小脑浦肯野细胞中有较高的水平。在这些神经元中，JSAP 1以微小颗粒染色形式存在于棘、树突、胞体和轴突中，通常与滑面内质网（sER）和细胞膜相关。免疫印迹显示富集分布在微粒体部分和胞质部分。因此，JSAP 1的特征性细胞表达和亚细胞分布可能有利于细胞有效地将外部刺激与JNK MAPK通路和其他细胞内机制联系起来。
The c-Jun N-terminal kinase (JNK) is one of the three major mitogen-activated protein kinases (MAPKs) playing key roles in various cellular processes in response to both extracellular and intracellular stimuli. JNK/SAPK-associated protein 1 (JSAP1 also referred to as JIP3) is a JNK-associated scaffold that controls the specificity and efficiency of JNK signaling cascades. Here we studied its expression in mouse brains. JSAP1 mRNA was expressed in developing and adult brains, showing spatial patterns similar to JNK1-3 mRNAs. In embryos, JSAP1 immunolabeling was intense for progenitor cells in the ventricular zone throughout the brain and in the external granular layer of the cerebellum, and for neurons and glial cells differentiating in the mantle zone. In adults, JSAP1 was distributed in various neurons and Bergmann glia, with higher levels in striatal cholinergic interneurons, telencephalic parvalbumin-positive interneurons and cerebellar Purkinje cells. In these neurons, JSAP1 was observed as tiny particulate staining in spines, dendrites, perikarya and axons, where it was often associated with the smooth endoplasmic reticulum (sER) and cell membrane. Immunoblots revealed enriched distribution in the microsomal fraction and cytosolic fraction. Therefore, the characteristic cellular expression and subcellular distribution of JSAP1 might be beneficial for cells to efficiently link external stimuli to the JNK MAPK pathway and other intracellular machineries.