Differentiated ovine tracheal epithelial cells support the colonisation of pathogenic and non-pathogenic strains of Mannheimia haemolytica.
Differentiated ovine tracheal epithelial cells support the colonisation of pathogenic and non-pathogenic strains of Mannheimia haemolytica.
复制标题
分化的绵羊气管上皮细胞支持致病性和非致病性溶血曼海姆菌菌株的定植。
DOI：
10.1038/s41598-020-71604-8
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发表时间：
2020
影响因子：
4.6
通讯作者：
O'Boyle N
中科院分区：
文献类型：
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作者：
O'Boyle N
溶血性曼氏杆菌是与反刍动物呼吸道疾病相关的主要细菌种。缺乏具有成本效益的、可重复的模型用于FM的研究。溶血性肺炎的发生阻碍了更好地了解控制疾病发展的分子相互作用的努力。我们采用高度优化的绵羊气管上皮细胞模型来评估各种致病性和非致病性M的定植。牛和羊源的溶血性分离株。通过组织相关细菌计数、组织学、免疫荧光显微镜和扫描电子显微镜对10个时间点上具有代表性的病原性和非病原性绵羊分离株进行比较，发现在黏附、增殖、细菌细胞生理和宿主细胞反应方面存在时间上的差异。在三个关键时间点(2小时、48小时和72小时)对8个牛和绵羊来源的菌株进行比较，发现定植并不是严格意义上的病原体或血清型特有的，A1、A2、A6和A12血清型的菌株能够在细胞层定植，而无论宿主种类或宿主的疾病状况。观察到病原性绵羊分离株有增加增殖能力的趋势。这些结果表明，棉铃虫的寄主专一性。溶血性感染可能至少部分是由于与定植相关的上皮界面处的黏附、侵袭和增殖过程造成的。
Mannheimia haemolyticais the primary bacterial species associated with respiratory disease of ruminants. A lack of cost-effective, reproducible models for the study ofM. haemolyticapathogenesis has hampered efforts to better understand the molecular interactions governing disease progression. We employed a highly optimised ovine tracheal epithelial cell model to assess the colonisation of various pathogenic and non-pathogenicM. haemolyticaisolates of bovine and ovine origin. Comparison of single representative pathogenic and non-pathogenic ovine isolates over ten time-points by enumeration of tissue-associated bacteria, histology, immunofluorescence microscopy and scanning electron microscopy revealed temporal differences in adhesion, proliferation, bacterial cell physiology and host cell responses. Comparison of eight isolates of bovine and ovine origin at three key time-points (2 h, 48 h and 72 h), revealed that colonisation was not strictly pathogen or serotype specific, with isolates of serotype A1, A2, A6 and A12 being capable of colonising the cell layer regardless of host species or disease status of the host. A trend towards increased proliferative capacity by pathogenic ovine isolates was observed. These results indicate that the host-specific nature ofM. haemolyticainfection may result at least partially from the colonisation-related processes of adhesion, invasion and proliferation at the epithelial interface.