Giant cells of the dorsal cochlear nucleus of mice: intracellular recordings in slices.
Giant cells of the dorsal cochlear nucleus of mice: intracellular recordings in slices.
复制标题
小鼠耳蜗背核的巨细胞：细胞内切片记录。
DOI：
10.1152/jn.1993.69.5.1398
复制
发表时间：
1993
影响因子：
2.5
通讯作者：
Oertel,D
中科院分区：
文献类型：
--
作者：
Zhang,S;Oertel,D
1.在小鼠耳蜗核复合体的切片中，从用生物胞素细胞内标记鉴定的五个巨细胞进行细胞内记录。巨细胞是耳蜗背核（DCN）的两条输出通路之一。了解神经元回路和内在电特性如何相互作用以控制巨细胞的放电是了解通过这些细胞传达的声学信息的一步。2.标记的巨细胞的胞体位于DCN的深层。树枝状晶沿着等轴和等轴分布广泛，主要分布在深层，但也有一些末端进入分子层。巨细胞的轴突较大，直径在1 ~ 2 μ m之间，穿过背侧声纹。在耳蜗核内未见分支。3.巨细胞发出大的、过冲的动作电位，随后是两个后超极化。第一个带来的膜电位低于休息，独立的强度注入电流。第二个变量更大，在动作电位之间的膜电位中产生下冲或拐点。4.在每五个标记的巨细胞，休克的神经根或前腹侧耳蜗核（AVCN）引起的单突触兴奋性突触后电位和两个串联的抑制性突触后电位（IPSPs）在第一个10毫秒。后来IPSPs随后laveling的10和50毫秒之间。单突触兴奋通常被缩短的抑制。5.士的宁，在1 μ M，阻止所有的IPSP在一个巨细胞测试，表明抑制输入这个巨细胞从电路固有的耳蜗核复合体是甘氨酸。6.两个巨细胞的传入神经的位置被映射。巨细胞的兴奋性和抑制性输入都可以由谷氨酸在AVCN和后腹侧耳蜗核中的许多位点的局部应用驱动，这表明腹侧耳蜗核VCN包含与巨细胞单突触或多突触连接的中间神经元。7.与结果一致的解释是，巨细胞由听觉神经纤维兴奋，由结核腹侧细胞抑制。巨细胞也可以被颗粒细胞或T星状细胞所兴奋。
1. In slices of the murine cochlear nuclear complex, intracellular recordings were made from five giant cells that were identified by intracellular labeling with biocytin. Giant cells form one of the two output pathways of the dorsal cochlear nucleus (DCN). Understanding how neuronal circuits and intrinsic electrical properties interact to control the firing of giant cells is a step toward understanding what acoustic information is conveyed through these cells. 2. Cell bodies of the labeled giant cells lay in the deep layer of the DCN. Dendrites, widespread both along the isofrequency axis and along the tonotopic axis, occupied mainly the deep layer, but some distal ends strayed into the molecular layer. Axons of giant cells were large, varying between 1 and 2 microns diam, and left through the dorsal acoustic stria. They were not observed to branch in the cochlear nuclei. 3. Giant cells fired large, overshooting action potentials that were followed by two afterhyperpolarizations. The first brought the membrane potential below rest, independent of the strength of injected current. The more variable second one produced either an undershoot or an inflection in the membrane potential between action potentials. 4. In each of the five labeled giant cells, shocks to the nerve root or to the anteroventral cochlear nucleus (AVCN) evoked a monosynaptic excitatory postsynaptic potential and two tandem inhibitory postsynaptic potentials (IPSPs) in the first 10 ms. Later IPSPs followed after latencies of between 10 and 50 ms. Monosynaptic excitation was usually cut short by the inhibition. 5. Strychnine, at 1 microM, blocked all IPSPs in the one giant cell tested, indicating that inhibitory input to this giant cell from circuits intrinsic to the cochlear nuclear complex was glycinergic. 6. The location of afferents was mapped for two giant cells. Both excitatory and inhibitory inputs to giant cells could be driven by the local application of glutamate to many loci in the AVCN and posteroventral cochlear nucleus, indicating that the ventral cochlear nucleus VCN contains interneurons that are monosynaptically or polysynaptically connected to giant cells. 7. An interpretation consistent with the results is that giant cells are excited by auditory nerve fibers and are inhibited by tuberculoventral cells. Giant cells may also be excited by granule or T stellate cells.