Cloning and expression of mouse cadherin-7, a type-II cadherin isolated from the developing eye
Cloning and expression of mouse cadherin-7, a type-II cadherin isolated from the developing eye
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DOI：
10.1006/mcne.1999.0764
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发表时间：
1999-07-01
影响因子：
3.5
通讯作者：
Tan, SS
中科院分区：
文献类型：
--
作者：
Faulkner-Jones, BE;Godinho, LNM;Tan, SS
我们报道了从小鼠胚胎眼组织中克隆钙粘蛋白-7。推导的氨基酸序列显示其为II型钙粘蛋白，类似于爪蟾F-cadherin和鸡钙粘蛋白-7。小鼠钙粘蛋白-7基因定位于1号染色体，位于8号染色体上钙粘蛋白基因的保守连锁群之外。钙粘蛋白-7在整个神经发育期间表达，并且mRNA水平在胚胎和出生后中枢神经系统（CNS）中都受到发育调节。在成年小鼠中，钙粘蛋白-7的表达仅限于CNS，在视网膜中的水平最高。在发育中的眼睛中，钙粘蛋白-7 mRNA仅在神经视网膜中发现。从E11开始，它由所有视网膜成神经细胞表达，但逐渐限于内部成神经细胞和发育中的神经节细胞层（GCL）中的神经元。在成人视网膜中，它仅限于GCL中的细胞亚群和内核层内部的无长突细胞。这种表达模式表明钙粘蛋白-7在小鼠视网膜发育中的作用，特别是在GCL的形成和维持中。
We report the molecular cloning of Cadherin-7 from the embryonic mouse eye. The deduced amino acid sequence shows it to be a type-II cadherin, similar to Xenopus F-cadherin and chick Cadherin-7. The mouse Cadherin-7 gene maps to chromosome 1, outside the conserved linkage group of cadherin genes on chromosome 8. Cadherin-7 is expressed throughout the entire period of neural development and mRNA levels are developmentally regulated in both the embryonic and the postnatal central nervous system (CNS). In adult mice, Cadherin-7 expression is restricted to the CNS, with highest levels in the retina. In the developing eye, Cadherin-7 mRNA is found only in the neural retina. It is expressed by all retinal neuroblasts from E11 onward, but becomes progressively restricted to neurons in the inner neuroblast and developing ganglion cell layers (GCL). In the adult retina it is confined to subpopulations of cells in the GCL and to amacrine cells in the inner part of the inner nuclear layer. This expression pattern suggests a role for Cadherin-7 in mouse retinal development, particularly in the formation and maintenance of the GCL.