HOST-CELL PROTEINS BIND TO THE CIS-ACTING SITE REQUIRED FOR VIRION-MEDIATED INDUCTION OF HERPES-SIMPLEX VIRUS-1 ALPHA-GENES
HOST-CELL PROTEINS BIND TO THE CIS-ACTING SITE REQUIRED FOR VIRION-MEDIATED INDUCTION OF HERPES-SIMPLEX VIRUS-1 ALPHA-GENES
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DOI：
10.1073/pnas.84.1.71
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发表时间：
1987-01-01
影响因子：
11.1
通讯作者：
ROIZMAN, B
中科院分区：
文献类型：
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作者：
KRISTIE, TM;ROIZMAN, B
单纯疱疹病毒I基因形成至少五组(α、β1、β2、γ1和γ2)，其表达以级联方式协调调节和顺序排列。在有效感染的细胞中，.alpha。基因首先表达，并且病毒体蛋白、α-反式诱导因子(α-TIF)以反式作用以增强其表达。 .alpha 的感应。通过α-TIF调节基因需要存在反式诱导顺式作用位点(α-TIC)，并且所有α-TIF的调节域中都包含α-TIC序列的一到三个同源物。基因。我们报道来自含有α-TIC同源物的α0、α4和α27基因的调节域的小DNA片段与宿主但不与病毒蛋白形成复合物。 DNA酶保护研究表明，在DNA凝胶阻滞测定中检测到的主要宿主蛋白复合物α-H1不对称地结合在α-TIC位点上。所有含有α-TIC同源物的DNA片段，但不包括那些缺乏同源物的DNA片段，竞争结合该复合物。其他宿主蛋白的结合位点的位置尚不清楚。含有α-TIC序列同源性的猿猴病毒40 DNA片段也与携带真正的α-TIC同源物的单纯疱疹病毒DNA片段竞争α-H1蛋白复合物。
The herpes simplex virus I genes form at least five groups (.alpha., .beta.1, .beta.2, .gamma.i, and .gamma.2) whose expression is coordinately regulated and sequentially ordered in a cascade fashion. In productively infected cells, the .alpha. genes are expressed first, and a virion protein, the .alpha.-trans-inducing factor (.alpha.-TIF), acts in trans to enhance their expression. Induction of the .alpha. genes by .alpha.-TIF requires the presence of a trans-induction cis-acting site (.alpha.-TIC), and one to three homologs of the .alpha.-TIC sequence are contained in the regulatory domains of all .alpha. genes. We report that small DNA fragments from regulatory domains of .alpha.0, .alpha.4, and .alpha.27 genes containing .alpha.-TIC homologs formed complexes with host but not viral proteins. DNase protection studies indicated that the major host protein complex .alpha.-H1 detected in DNA gel retardation assays bound asymmetrically across the .alpha.-TIC site. All DNA fragments containing .alpha.-TIC homologs, but not those lacking the homolog, competed for the binding of this complex. The location of the binding site of the other host proteins is not yet known. Simian virus 40 DNA fragments containing a homology of the .alpha.-TIC sequence also competed with herpes simplex virus DNA fragmnents carrying authentic .alpha.-TIC homologs for the .alpha.-H1 protein complex.