THIOLATION OF TRANSFER-RNA IN ESCHERICHIA-COLI VARIES WITH GROWTH-RATE
THIOLATION OF TRANSFER-RNA IN ESCHERICHIA-COLI VARIES WITH GROWTH-RATE
复制标题
DOI：
10.1093/nar/20.17.4499
复制
发表时间：
1992-09-11
影响因子：
14.9
通讯作者：
KURLAND, CG
中科院分区：
文献类型：
--
作者：
EMILSSON, V;NASLUND, AK;KURLAND, CG
我们使用了一种亲和电泳试验，当与北方杂交技术相结合时，使我们能够估计大肠杆菌中单个tRNA种类的巯基化程度。我们观察到，4-硫代2 '（3'）-尿苷（4-硫代U）的水平在许多但不是所有的tRNA显着变化在不同的细菌生长速率：五个tRNA是完全硫醇化在所有的生长速率，而另外八个tRNA是不完全硫醇化和这些tRNA物种的未硫醇化形式的分数增加，随着生长速率的增加。转移RNA 2Glu含有4-硫代U以及（甲氨基）甲基-2-硫代尿苷（mnm（5）2-硫代U）。tRNA 2Glu的mnm-（5）2-thiouU水平与生长速率无关。令人惊讶的是，我们研究的13种tRNA中没有一种在所有生长培养基中完全未被修饰。特别是，在最慢的增长率，我们研究的每一个tRNA类包含一个形式，有4-硫代U残基。
We have used an affinity electrophoresis assay which when combined with Northern hybridization techniques permits us to estimate the degree of thiolation of individual tRNA species in Escherichia coli. We observe that the levels of 4-thio 2'(3')-uridine (4-thioU) in many but not all tRNAs varies dramatically at different bacterial growth rates: Five tRNAs are completely thiolated at all growth rates, while another eight tRNAs are incompletely thiolated and the fraction of the unthiolated form of these tRNA species increases as the growth rates increase. Transfer RNA2Glu contains 4-thioU as well as (methylamino)methyl-2-thio uridine (mnm(5)2-thioU). The level of mnm-(5)2-thioU of tRNA2Glu is invariant with growth rate. Surprisingly, none of the thirteen tRNA species that we have studied is completely unmodified in all growth media. In particular, at the slowest growth rates every tRNA class that we have studied contains a form that has 4-thioU residues.