Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina
Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina
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DOI：
10.1007/s00418-011-0853-8
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发表时间：
2011-08
影响因子：
2.3
通讯作者：
T. Wakabayashi;Tetsuji Mori;Y. Hirahara;T. Koike;Y. Kubota;Y. Takamori;H. Yamada
中科院分区：
文献类型：
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作者：
T. Wakabayashi;Tetsuji Mori;Y. Hirahara;T. Koike;Y. Kubota;Y. Takamori;H. Yamada
核纤层蛋白是支持核膜的V型中间丝蛋白。它们分为A型核纤层蛋白，包括核纤层蛋白A和C，以及B型核纤层蛋白，包括核纤层蛋白B1和B2。在大鼠脑中，核纤层蛋白A和C以相对相等的量表达，而核纤层蛋白B1和B2的表达根据细胞类型而变化。核纤层蛋白在正常形态发生和功能中起重要作用。在神经系统中，它们的异常表达导致几种神经退行性疾病，如周围神经病变、脑白质营养不良和无脑畸形。视网膜属于中枢神经系统（CNS），并且已被广泛用作CNS神经元的来源。我们通过免疫组化研究了成年大鼠视网膜中核纤层蛋白亚型的表达模式，发现与大脑相比，染色模式不同。所有视网膜神经元表达核纤层蛋白B1和B2的相对等量。此外，水平细胞和视网膜神经节细胞亚群表达核纤层蛋白A和C，而感光细胞既不表达核纤层蛋白A也不表达核纤层蛋白C，所有其他视网膜神经元仅表达核纤层蛋白C。核纤层蛋白在视网膜神经元中的这种差异表达模式表明它们可能参与单个视网膜神经元中的细胞分化和细胞特异性基因的表达。
Lamins are type V intermediate filament proteins that support nuclear membranes. They are divided into A-type lamins, which include lamin A and C, and B-type lamins, which include lamin B1 and B2. In the rat brain, lamin A and C are expressed in relatively equal amounts, while the expressions of lamin B1 and B2 vary depending on the cell type. Lamins play important roles in normal morphogenesis and function. In the nervous system, their abnormal expression causes several neurodegenerative diseases such as peripheral neuropathy, leukodystrophy and lissencephaly. The retina belongs to the central nervous system (CNS) and has widely been used as a source of CNS neurons. We investigated the expression patterns of lamin subtypes in the adult rat retina by immunohistochemistry and found that the staining patterns differed when compared with the brain. All retinal neurons expressed lamin B1 and B2 in relatively equal amounts. In addition, horizontal cells and a subpopulation of retinal ganglion cells expressed lamin A and C, while photoreceptor cells expressed neither lamin A nor C, and all other retinal neurons expressed lamin C only. This differential expression pattern of lamins in retinal neurons suggests that they may be involved in cellular differentiation and expression of cell-specific genes in individual retinal neurons.