PHOSPHORYLATION OF BACTERIAL RESPONSE REGULATOR PROTEINS BY LOW-MOLECULAR-WEIGHT PHOSPHO-DONORS
PHOSPHORYLATION OF BACTERIAL RESPONSE REGULATOR PROTEINS BY LOW-MOLECULAR-WEIGHT PHOSPHO-DONORS
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DOI：
10.1073/pnas.89.2.718
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发表时间：
1992-01-15
影响因子：
11.1
通讯作者：
STOCK, JB
中科院分区：
文献类型：
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作者：
LUKAT, GS;MCCLEARY, WR;STOCK, JB
细菌的运动和基因表达由磷酸化反应调节剂家族控制，其活性由相关的蛋白组氨酸激酶家族调节。在趋化作用中，有两种反应调节剂 CheY 和 CheB，它们接收来自组氨酸激酶 CheA 的磷酰基。在这里，我们表明，反应调节剂催化其自身的磷酸化，因为 CheY 和 CheB 都可以在完全不存在任何辅助蛋白的情况下被磷酸化。 CheY 和 CheB 均使用氨基磷酸酯 (NH2PO32-) 中的 N-磷酰基作为磷酸供体。这种酶活性可能反映了反应调节剂从其相关激酶中的磷酸组氨酸接受磷酰基的一般能力。它为在没有激酶蛋白的情况下研究激活的反应调节剂提供了一种通用方法。 CheY 还可以使用中间代谢物，如乙酰磷酸和氨基甲酰磷酸作为磷酸供体。这些反应可能提供一种机制来调节细胞行为以响应代谢状态的改变。
Bacterial motility and gene expression are controlled by a family of phosphorylated response regulators whose activities are modulated by an associated family of protein-histidine kinases. In chemotaxis there are two response regulators, CheY and CheB, that receive phosphoryl groups from the histidine kinase, CheA. Here we show that the response regulators catalyze their own phosphorylation in that both CheY and CheB can be phosphorylated in the complete absence of any auxiliary protein. Both CheY and CheB use the N-phosphoryl group in phosphoramidate (NH2PO32-) as a phospho-donor. This enzymatic activity probably reflects the general ability of response regulators to accept phosphoryl groups from phosphohistidines in their associated kinases. It provides a general method for the study of activated response regulators in the absence of kinase proteins. CheY can also use intermediary metabolites such as acetyl phosphate and carbamoyl phosphate as phospho-donors. These reactions may provide a mechanism to modulate cell behavior in response to altered metabolic states.