METABOLISM OF BENZIDINE-BASED DYES AND THE APPEARANCE OF MUTAGENIC METABOLITES IN URINE OF RATS AFTER ORAL OR INTRAPERITONEAL ADMINISTRATION
METABOLISM OF BENZIDINE-BASED DYES AND THE APPEARANCE OF MUTAGENIC METABOLITES IN URINE OF RATS AFTER ORAL OR INTRAPERITONEAL ADMINISTRATION
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DOI：
10.1016/0300-483x(84)90108-2
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发表时间：
1984-01-01
期刊：
影响因子：
4.5
通讯作者：
HENDERSON, PT
中科院分区：
文献类型：
--
作者：
BOS, RP;GROENEN, MAM;HENDERSON, PT
研究了3种偶氮染料的体外还原、乙酰化和体内诱变活性。在有大鼠肝脏存在的情况下，直接黑38和直接棕95的上清释放出联苯胺9000 g，而直接蓝6孵育期间几乎不产生联苯胺。联苯胺与分离的大鼠肝细胞孵育产生二乙酰联苯胺。除非在培养液中加入乙酰化反应的辅助因子乙酰辅酶a，否则联苯胺与大鼠肝脏9000 g上清液孵育过程中不会形成二乙酰联苯胺。在不添加乙酰辅酶a的情况下，分离的大鼠肝细胞能够从直接黑38、直接蓝6或直接棕95中产生二乙酰联苯胺。给大鼠注射联苯胺、直接黑38或直接棕95后，尿中出现突变性。直接口服黑38的尿致突变性值明显高于内服药。联苯胺和直接棕95没有观察到这种差异。大鼠肝脏具有相当大的还原偶氮化合物的能力。对于一些化合物，如直接黑38，肝外酶，最有可能存在于肠道菌群中，也可能在偶氮切割中发挥重要作用。
The azo reduction and acetylation in vitro and the mutagenic activation in vivo of 3 azo dyes were studied. In the presence of rat liver 9000 g supernatant benzidine was released from direct black 38 and direct brown 95, whereas hardly any benzidine was produced during incubation of direct blue 6. Incubation of benzidine with isolated rat hepatocytes resulted in the appearance of diacetylbenzidine. No diacetylbenzidine was formed during incubation of benzidine with rat liver 9000 g supernatant, unless the cofactor for the acetylation reaction, acetyl CoA, was added to the incubation medium. Isolated rat hepatocytes were capable to produce diacetylbenzidine from direct black 38, direct blue 6 or direct brown 95 without supplementation with acetyl CoA. Administration of benzidine, direct black 38 or direct brown 95 to rats resulted in the appearance of mutagenicity in urine. For direct black 38 significantly higher mutagenicity values were found in urine after oral administration than after i.p. treatment. Such differences were not observed for benzidine and direct brown 95. Rat liver has a considerable capacity to reduce azo compounds. For some compounds, like direct black 38, extrahepatic enzymes, most likely present in the intestinal flora, may also play a substantial role in the azo cleavage.