Immune response in humans after vaccination with vaccinia virus: generation of a virus-specific cytotoxic activity by human peripheral lymphocytes.
Immune response in humans after vaccination with vaccinia virus: generation of a virus-specific cytotoxic activity by human peripheral lymphocytes.
复制标题
疫苗病毒接种后人类的免疫反应：人类外周淋巴细胞产生病毒特异性细胞毒性活性。
DOI：
10.1084/jem.146.4.949
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发表时间：
1977-10-01
影响因子：
15.3
通讯作者：
Oldstone, M B
中科院分区：
文献类型：
--
作者：
Perrin, L H;Zinkernagel, R M;Oldstone, M B
在人类志愿者接种痘苗病毒疫苗后，10天内出现局部硬结，并在一半人中检测到区域性腺病。免疫后不同时间采集的外周血淋巴细胞(PBL)对痘苗病毒感染的靶细胞有较高的杀伤活性，在第7天达到高峰。细胞毒活性的特异性与人类白细胞抗原标志无关，因为自体、同种和异种感染的靶细胞的裂解效率相同。这种细胞毒活性是由PBL引起的，这种PBL不与绵羊红细胞花环，可以通过去除Fc受体携带细胞而被耗尽90%以上。去除T细胞的PBL显示出比完整PBL高一半到两倍的细胞毒性。兔抗人免疫球蛋白Fab(2)也可使其细胞毒活性降低95%以上。另一方面，非免疫性PBL在有针对痘苗病毒的特异性抗体存在的情况下裂解痘苗病毒感染的靶细胞，从而证明ADCC可以有效地裂解痘苗病毒感染的靶细胞。我们的结论是，疫苗接种后，抗体形成细胞产生并提供特异性的抗病毒抗体，在该反应中检测到的细胞毒细胞是K细胞。这些实验表明，抗体依赖的细胞毒性可能在人类对病毒感染的恢复中起着重要作用。
After vaccinia virus vaccination of human volunteers, local indurations developed within 10 days, and regional adenopathy was detected in half of the individuals. Their peripheral blood lymphocytes (PBL) harvested at different days after vaccination showed specific activity against target cells infected with vaccinia virus with a peak activity at day 7. The specificity of the cytotoxic activity was not related to HLA markers, since autologous, homologous, and heterologous infected target cells were lysed with the same efficiency. The cytotoxic activity was caused by PBL that did not rosette with sheep erythrocytes and could be depleted by more than 90 percent by removing Fc receptor-bearing cells. T-cell- depleted PBL showed a one-half to two times greater cytotoxicity than intact PBL. The cytotoxic activity could also be abrogated by more than 95 percent by rabbit Fab(2) anti-human IgG. On the other hand, nonimmune PBL lysed vaccinia-infected target cells in the presence of specific antibodies against vaccinia virus, thus demonstrating that ADCC could be efficient in lysing vaccinia-infected target cells. We conclude that after vaccination, antibody-forming cells arise and provide specific anti-viral antibody and that the cytotoxic cells detected in this reaction are K cells. These experiments suggest that antibody-dependent cell cytotoxicity may be of major importance in the recovery of man to virus infections.