Synthesis of infectious human papillomavirus type 18 in differentiating epithelium transfected with viral DNA
Synthesis of infectious human papillomavirus type 18 in differentiating epithelium transfected with viral DNA
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DOI：
10.1128/jvi.71.10.7381-7386.1997
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发表时间：
1997-10-01
影响因子：
5.4
通讯作者：
Ozbun, MA
中科院分区：
文献类型：
--
作者：
Meyers, C;Mayer, TJ;Ozbun, MA
缺乏一个允许系统的病毒股票含有丰富的人乳头瘤病毒（HPV）颗粒的繁殖，阻碍了感染性和HPV复制的早期阶段的研究，器官型（筏）培养系统允许HPV的分化的具体方面，包括病毒DNA的扩增，晚期基因的表达，和病毒形态发生的研究。然而，这些研究仅限于单一的病毒类型，即HPV 31型（HPV 31）。我们通过电穿孔将线性化的HPV 18基因组DNA人工引入原代角质形成细胞，随后在器官型培养中进行克隆扩增和诱导上皮分层和分化。本文报道人乳头瘤病毒18型（HPV18）感染性病毒粒子的合成。通过等密度梯度纯化的HPV 18病毒粒子能够在体外感染角质形成细胞，如通过表达多个HPV 18特异性剪接转录物所示。
The lack of a permissive system for the propagation of viral stocks containing abundant human papillomavirus (HPV) particles has hindered the study of infectivity and the early stages of HPV replication, The organotypic (raft) culture system has permitted the study of a number of the differentiation specific aspects of HPV, including amplification of viral DNA, expression of late genes, and viral morphogenesis. However, these investigations have been limited to a single virus type, namely, HPV type 31 (HPV31), We have artificially introduced linearized HPV18 genomic DNA into primary keratinocytes by electroporation, followed by clonal expansion and induction of epithelial stratification and differentiation in organotypic culture. We report the synthesis of infectious HPV18 virions, Virus particles approximately 50 nm in diameter were observed by electron microscopy. HPV18 virions purified by isopycnic gradient were capable of infecting keratinocytes in vitro, as shown by the expression of multiple HPV18-specific, spliced transcripts.