Proliferation of germ cells during gonadal sex differentiation in medaka:: Insights from germ cell-depleted mutant zenzai
Proliferation of germ cells during gonadal sex differentiation in medaka:: Insights from germ cell-depleted mutant zenzai
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DOI：
10.1016/j.ydbio.2007.07.039
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发表时间：
2007-10-15
影响因子：
2.7
通讯作者：
Tanaka, Minoru
中科院分区：
文献类型：
--
作者：
Saito, Daisuke;Morinaga, Chikako;Tanaka, Minoru
生殖细胞的增殖在性腺性别分化期间变得性二态，尽管脊椎动物对此的潜在动力学还没有很好的了解。通过跟踪GFP标记的生殖细胞在体内和分析的生殖细胞耗尽的突变体，zenzai，我们表明，生殖细胞的增殖和分化的调节在硬骨鱼青鳉性二态的方式。在未分化的性腺中，生殖细胞通过缓慢的间歇性分裂（1型）恢复增殖，产生分离的子细胞。当雄性生殖腺中的生殖细胞继续这种增殖模式时，雌性生殖腺中的一些生殖细胞组分开始两到四轮连续分裂（11型），形成四个，八个或十六个细胞的包囊，随后同步进入减数分裂。因此，雌性生殖细胞比雄性生殖细胞更早分化。在zenzai突变体中，缓慢间歇性分裂的缺陷最终导致两性成年性腺中生殖细胞的耗尽，尽管事实上囊肿形成分裂不受影响。这表明，缓慢的间歇性分裂对于生殖细胞的维持至关重要。生殖细胞的增殖和分化是脊椎动物性腺性别分化的重要组成部分。(C)2007年爱思唯尔公司All rights reserved.
The proliferation of germ cells becomes sexually dimorphic during gonadal sex differentiation, although the underlying dynamics of this are not well understood in vertebrates. By tracing GFP-labeled germ cells in vivo and analyzing the germ cell-depleted mutant, zenzai, we show that the proliferation and differentiation of germ cells are regulated in a sexually dimorphic manner in the teleost fish medaka. In the undifferentiated gonads, germ cells resume proliferation by slow intermittent division (type 1), producing isolated daughter cells. While germ cells in the male gonads continue this mode of proliferation, some germ cell fractions in the female gonads initiate two to four rounds of continuous division (type 11), forming cysts of four, eight, or sixteen cells, which subsequently enter meiosis synchronously. Thus, female germ cells become differentiated much earlier than do male germ cells. In the zenzai mutant, a defect in slow intermittent division eventually leads to the depletion of germ cells in the adult gonads in both sexes, despite the fact that cyst-forming division is unaffected. This argues that slow intermittent division is essential for the maintenance of germ cells. The proliferation and differentiation of germ cells are thus important components of gonadal sex differentiation in vertebrates. (C) 2007 Elsevier Inc. All rights reserved.