Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors
Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors
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DOI：
10.1038/nm981
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发表时间：
2004-02-01
期刊：
影响因子：
82.9
通讯作者：
Jain, RK
中科院分区：
文献类型：
--
作者：
Alexandrakis, G;Brown, EB;Jain, RK
转运参数决定了药物进入肿瘤的途径。然而，技术上的困难阻碍了活体组织深处这些参数的测量。为此，我们采用并进一步优化了双光子荧光相关显微镜（TPFCM），用于肿瘤中传输参数的体内测量。TPFCM将肿瘤中扩散系数的可检测范围扩展了一个数量级，并揭示了扩散的快速和缓慢成分。这两种组分的比例取决于分子大小，并且可以在体内用透明质酸酶和胶原酶改变。这些研究表明，TPFCM是一种很有前途的工具，解剖的障碍，药物输送在肿瘤。
Transport parameters determine the access of drugs to tumors. However, technical difficulties preclude the measurement of these parameters deep inside living tissues. To this end, we adapted and further optimized two-photon fluorescence correlation microscopy (TPFCM) for in vivo measurement of transport parameters in tumors. TPFCM extends the detectable range of diffusion coefficients in tumors by one order of magnitude, and reveals both a fast and a slow component of diffusion. The ratio of these two components depends on molecular size and can be altered in vivo with hyaluronidase and collagenase. These studies indicate that TPFCM is a promising tool to dissect the barriers to drug delivery in tumors.