In vivo skin optical clearing by glycerol solutions: mechanism
In vivo skin optical clearing by glycerol solutions: mechanism
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甘油溶液体内皮肤光学透明化：机制
DOI：
10.1002/jbio.200910080
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Zhu, Dan
中科院分区：
文献类型：
--
作者：
Wen, Xiang;Mao, Zongzhen;Zhu, Dan
光在生物组织中的穿透性可以通过使用光学透明技术来增强。然而，对其作用机制的研究仅限于体外实验。本研究以大鼠背部皮肤为实验材料，探讨其体内透光机制。经皮注射不同浓度的甘油溶液，监测皮肤反射光谱、倍频成像和显微结构的变化。结果表明，随着皮肤变得透明，相应的反射率降低，真皮厚度和胶原纤维直径减小，但没有胶原纤维溶解或断裂。因此，真皮厚度的减少和相应的组织纤维的规整堆积在甘油诱导的体内皮肤光学透明的机制中起着重要的作用。[图]在注射(A)空白对照；(B)20%-甘油；(C)30%-甘油；(D)75%-甘油；(x30000，比例=333 nm)10min后，用透射电子显微镜拍摄真皮胶原纤维的图像。(C)2010年，Wiley-VCH Verlag GmbH&Co.KGaA。温海姆
The penetration of light in biological tissues can be enhanced by using optical-clearing techniques. However, researches on the mechanism are limited to in vitro experiments. In this study, rat dorsal skin was used to discuss the mechanism for in vivo optical clearing. Glycerol solutions with different concentrations were applied by dermal injection; then the skin reflectance spectrum, SHG imaging and microstructural changes were monitored. The results showed that with the skin becoming transparent, the corresponding reflectance decreased, and the thickness of dermis and diameter of collagen fibril decreased, but no collagen fiber was dissolved or fractured. Hence, it can be concluded that the thickness decrease of dermis and corresponding more regular packing of tissue fibers plays an important role in the mechanism for glycerol-induced optical clearing of skin in vivo.[GRAPHICS]Images of dermal collagen fibers taken by TEM 10 min after injection of (a) blank control; (b) 20%-glycerol; (c) 30%-glycerol; (d) 75%-glycerol; (x30000, scale = 333 nm). (C) 2010 by WILEY-VCH Verlag GmbH & Co. KGaA. Weinheim