Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
Use of DNA microarray analysis in diagnosis of bacterial and fungal endophthalmitis
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DOI：
10.2147/opth.s29230
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发表时间：
2012-01-01
影响因子：
2.2
通讯作者：
Shimadzu, Mitsunobu
中科院分区：
文献类型：
--
作者：
Sakai, Tsutomu;Kohzaki, Kenichi;Shimadzu, Mitsunobu
背景资料：目的：探讨DNA微阵列分析用于鉴定眼内炎致病微生物的实用性。方法：收集13例玻璃体切除术患者的13份玻璃体液标本。玻璃体液从3例疑似眼内炎和10个对照组无感染进行测试的存在下，细菌和真菌的培养试验，聚合酶链反应（PCR）分析，和DNA微阵列analysis.Results：没有控制样品是阳性的细菌或真菌的培养试验，PCR，或微阵列analysis.Results。两名疑似眼内炎患者（病例1和2）的标本在PCR中对细菌呈阳性，一名患者（病例3）的标本在PCR中对真菌呈阳性。PCR阳性标本中肺炎克雷伯菌（病例1）、无乳链球菌（病例2）和近平滑念珠菌（病例3）在24小时内通过DNA微阵列分析鉴定。培养结果也为阳性K。肺炎病例1，S.病例2为无乳梭菌C.近平滑的情况下，3，但需要3至4天获得。结论：微阵列分析是补充常规文化确定致病微生物，并可能是一个有用的工具，在患者怀疑眼内炎，需要快速诊断和早期抗生素治疗。
Background: To examine the utility of DNA microarray analysis for identifying causative microorganisms in endophthalmitis.Methods: Thirteen samples of vitreous fluid (VF) were obtained from 13 patients during - vitrectomy. Vitreous fluids from three patients with suspected endophthalmitis and ten controls without infection were subjected to testing for the presence of bacteria and fungi in culture tests, polymerase chain reaction (PCR) analysis, and DNA microarray analysis.Results: No control sample was positive for bacteria or fungi in the culture test, PCR, or microarray analysis. Specimens from two patients (Cases 1 and 2) with suspected endophthalmitis were positive for bacteria in PCR, and a specimen from one patient (Case 3) was positive for fungi in PCR. Klebsiella pneumonia (Case 1), Streptococcus agalactiae (Case 2), and Candida parapsilosis (Case 3) in the PCR-positive specimens were identified by DNA microarray analysis within 24 hours. Culture results were also positive for K. pneumonia in Case 1, S. agalactiae in Case 2, and C. parapsilosis in Case 3, but required 3 to 4 days to obtain.Conclusions: Microarray analysis is complementary to routine cultures for identifying causative microorganisms and is likely to be a useful tool in patients with suspected endophthalmitis who require rapid diagnosis and early antibiotic treatment.