Activation of the hedgehog pathway in a subset of lung cancers
Activation of the hedgehog pathway in a subset of lung cancers
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DOI：
10.1016/j.canlet.2005.11.036
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发表时间：
2006-11-28
期刊：
影响因子：
9.7
通讯作者：
Xie, Jingwu
中科院分区：
文献类型：
--
作者：
Chi, Sumin;Huang, Shuhong;Xie, Jingwu
据报道，刺猬蛋白通路在肺癌中被激活，但其频率仍然未知。我们通过原位杂交和免疫组织化学检查了肺癌中该通路的激活情况，发现不到 10% 的肿瘤具有刺猬蛋白靶基因表达升高。我们进一步鉴定了细胞系 NCI-H209 和两个原发性肿瘤，未检测到 Su(Fu)（该途径的负调节因子）。 NCI-H209细胞中Su(Fu)的异位表达下调hedgehog靶基因表达并导致细胞增殖抑制。这些数据表明，hedgehog通路的激活仅在一部分肺癌中通过Shh过度表达或Su(Fu)失活而被激活。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
Activation of the hedgehog pathway is reported in lung cancer, but its frequency remains unknown. We examine activation of this pathway in lung cancers by in situ hybridization and immunohistochemistry, and find that less than 10% of the tumors have elevated hedgehog target gene expression. We further identify a cell line NCI-H209 and two primary tumors with no detectable Su(Fu), a negative regulator of the pathway. Ectopic expression of Su(Fu) in NCI-H209 cells down-regulates hedgehog target gene expression and leads to inhibition of cell proliferation. These data indicate that activation of the hedgehog pathway is activated through Shh over-expression or Su(Fu) inactivation in only a subset of lung cancers. (c) 2005 Elsevier Ireland Ltd. All rights reserved.