Utility of 123C3 monoclonal antibody against CD56 (NCAM) for the diagnosis of small cell carcinomas on paraffin sections
Utility of 123C3 monoclonal antibody against CD56 (NCAM) for the diagnosis of small cell carcinomas on paraffin sections
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DOI：
10.1016/s0046-8177(97)90226-4
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发表时间：
1997-12-01
期刊：
影响因子：
3.3
通讯作者：
Dietel, M
中科院分区：
文献类型：
--
作者：
Kaufmann, O;Georgi, T;Dietel, M
CD 56在几乎所有小细胞癌的冷冻切片上都是免疫化学检测到的。作者回顾性检测了抗CD 56单克隆抗体123 C3在抗原修复后通过石蜡切片免疫组织化学区分肺和肺外小细胞癌与非神经内分泌非小细胞癌的有效性。该研究包括70例小细胞癌和344例原发性和转移性非神经内分泌癌的不同原发部位。将染色结果与特异性神经内分泌标志物（CD 57、嗜铬粒蛋白A、突触素）进行比较。单克隆抗体123 C3对大多数小细胞癌进行弥漫性染色，具有强膜性模式（灵敏度：0.99）。在有挤压伪影的区域或脱钙后，染色强度未减弱。神经内分泌标志物对小细胞癌的联合敏感性仅为0.44。关于非神经内分泌癌，123 C3抗体染色7/28卵巢癌，6/30肾细胞癌，2/10子宫内膜癌，2/3肺非神经内分泌大细胞癌，1/38腺癌，4/52肺鳞状细胞癌。尿路上皮癌，肝细胞癌，鳞状细胞癌的头部，颈部和cenix子宫，以及腺癌的乳腺癌，胃，结肠，胰腺和前列腺，显示没有免疫反应性的CD 56。123 C3和联合神经内分泌标志物对小细胞癌的特异性分别为0.94和0.95。作者得出结论，单克隆抗体123 C3可能有助于石蜡切片上小细胞癌与非神经内分泌癌的免疫组织化学鉴别，特别是在小的和压碎的活检标本中。版权所有（C）1997 W.B.桑德斯公司
CD56 is immunohistochemically detectable in virtually all small cell carcinomas on frozen sections. The authors retrospectively tested the usefulness of the monoclonal antibody 123C3 against CD56 to differentiate pulmonary and extrapulmonary small cell carcinomas from nonneuroendocrine non-small cell carcinomas by paraffin-section immunohistochemistry after antigen retrieval. The study included 70 small cell carcinomas and 344 primary and metastatic nonneuroendocrine carcinomas of various primary sites. The staining results were compared with specific neuroendocrine markers (CD57, Chromogranin A, Synaptophysin). The monoclonal antibody 123C3 diffusely stained most small cell carcinomas with a strong membranous pattern (sensitivity: 0.99). The staining intensity was not diminished in areas with crush artifacts or after decalcification. The neuroendocrine markers had a combined sensitivity of only 0.44 for small cell carcinomas. With regard to nonneuroendocrine carcinomas, the 123C3 antibody stained 7 of 28 ovarian carcinomas, 6 of 30 renal cell carcinomas, 2 of 10 endometrial carcinomas, two of three nonneuroendocrine large cell carcinomas of the lung, 1 of 38 adenocarcinomas, and 4 of 52 squamous cell carcinomas of the lung. Urothelial carcinomas, hepatocellular carcinomas, squamous carcinomas of the head,neck and cenix uteri, as well as adenocarcinomas of the breast, stomach, colon, pancreas, and prostate, showed no immunoreactivity for CD56. The specificities of 123C3 and the combined neuroendocrine markers for small cell carcinomas were 0.94 and 0.95, respectively. The authors conclude that monoclonal antibody 123C3 might be useful for the immunohistochemical differentiation of small cell carcinomas from nonneuroendocrine carcinomas on paraffin sections, especially in small and crushed biopsy specimens. Copyright (C) 1997 by W.B. Saunders Company.