Gonadotrophin in the term placenta of the dolphin (Tursiops truncatus), the Californian sea lion (Zalophus californianus), the grey seal (Halichoerus grypus) and man.
Gonadotrophin in the term placenta of the dolphin (Tursiops truncatus), the Californian sea lion (Zalophus californianus), the grey seal (Halichoerus grypus) and man.
复制标题
海豚 (Tursiops truncatus)、加州海狮 (Zalophus californianus)、灰海豹 (Halichoerus grypus) 和人类胎盘中的促性腺激素。
DOI：
10.1530/jrf.0.0760637
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
L. Wide
中科院分区：
文献类型：
--
作者：
B. Hobson;L. Wide
采用hCG作为免疫原和标记抗原的固相放射免疫法测定了海豚、海狮和灰海豹胎盘提取物中绒毛膜促性腺激素的活性，并对其增加小鼠子宫重量的效果进行了测定。在这些胎盘中发现了生物反应性(B)促性腺激素，与人类胎盘相比，海豚胎盘中的CG浓度较高，海狮胎盘中的CG浓度相似，灰海豹胎盘中的CG浓度较低。用兔抗绒毛膜促性腺激素血清中和各物种的生物活性。所有胎盘提取物均含有hCG免疫测定活性物质(I)。海洋哺乳动物胎盘提取物中CG的B/I比显著高于人足月胎盘。体内生物测定、RIA、电泳和凝胶色谱研究结果表明，海洋哺乳动物胎盘中CG的结构与人类胎盘中CG的结构相似。
Chorionic gonadotrophin activity in extracts of the term placenta of a dolphin, a sea lion and a grey seal was measured by its effectiveness in increasing uterine weight in the mouse and by solid-phase RIA using hCG as immunogen and labelled antigen. Bioreactive (B) gonadotrophin was found in these placentae and, compared to the human term placenta, the concentration of CG in the dolphin was higher, in the sea lion similar and in the grey seal lower. The biological activity in each species was neutralized with a rabbit anti-hCG serum. All placental extracts contained material active in the hCG immunoassay (I). The ratio B/I was significantly higher for the CG in the placental extracts of the marine mammals compared with that of the human term placenta. Results of in-vivo bioassay, RIA, electrophoretic and gel-chromatographic studies indicate structural similarities between CG in the placentae of the marine mammals and human CG.