Isolation and characterization of a novel potato Auxin/Indole-3-Acetic Acid family member (StIAA2) that is involved in petiole hyponasty and shoot morphogenesis
Isolation and characterization of a novel potato Auxin/Indole-3-Acetic Acid family member (StIAA2) that is involved in petiole hyponasty and shoot morphogenesis
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DOI：
10.1016/j.plaphy.2006.10.026
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发表时间：
2006-11-01
影响因子：
6.5
通讯作者：
Bachem, C. W. B.
中科院分区：
文献类型：
--
作者：
Kloosterman, B.;Visser, R. G. F.;Bachem, C. W. B.
生长素/吲哚-3-乙酸（Aux/IAA）蛋白是一种短寿命的转录调节因子，通过与生长素反应因子（ARE）的相互作用来介导其反应。虽然已经在拟南芥中鉴定了29个Aux/IAA蛋白，但它们的个体功能仍然知之甚少，并且主要由功能获得性突变等位基因中观察到的生长缺陷来定义。在这里，我们提出了一种新的Aux/IAA蛋白在马铃薯（Solanum tuberosum），命名为StIAA 2的分离和表征。StIAA 2的下调导致独特的表型，包括增加的植株高度、叶柄低能性和茎尖中生长的叶原基的极端弯曲。StIAA 2转录水平降低的转基因植物的基因表达分析导致鉴定出许多表达谱改变的基因，包括Aux/IAA基因家族（StIAA）的另一个成员。在StIAA 2抑制克隆中观察到的表型可以与Aux/IAA家族成员之间的共同以及独特的功能作用相关联，这表明分析Aux/IAA在不同植物物种中表达的重要性。(c)2006年爱思唯尔马森SAS。All rights reserved.
Auxin/indole-3-acetid acid (Aux/IAA) proteins are short-lived transcriptional regulators that mediate their response through interaction with auxin response factors (ARE). Although 29 Aux/IAA proteins have been identified in Arabidopsis thaliana, their individual functions are still poorly understood and are largely defined by observed growth defects in gain-of-function mutant alleles. Here we present the isolation and characterization of a novel Aux/IAA protein in potato (Solanum tuberosum) that is named StIAA2. Down regulation of StIAA2 results in distinctive phenotypes that include, increased plant height, petiole hyponasty and extreme curvature of growing leaf primordia in the shoot apex. Gene expression analysis of transgenic plants with reduced StIAA2 transcript levels resulted in the identification of a number of genes with altered expression profiles including another member of the Aux/IAA gene family (StIAA). The phenotypes that were observed in the StIAA2 suppression clones can be associated with both common as well as unique functional roles among Aux/IAA family members indicating the importance of analyzing Aux/IAA expression in different plant species. (c) 2006 Elsevier Masson SAS. All rights reserved.