Genotoxicity testing of two lead-compounds in somatic cells of Drosophila melanogaster
Genotoxicity testing of two lead-compounds in somatic cells of Drosophila melanogaster
复制标题
DOI：
10.1016/j.mrgentox.2011.05.008
复制
发表时间：
2011-09-18
影响因子：
1.9
通讯作者：
Marcos, Ricard
中科院分区：
文献类型：
--
作者：
Carmona, Erico R.;Creus, Amadeu;Marcos, Ricard
通过测定两个不同的遗传终点，研究了两种无机铅化合物在果蝇体内的遗传毒性活性。我们使用了翅斑试验和彗星试验。用幼虫血细胞进行彗星试验。翅斑试验的结果表明，氯化铅、PbCl 2和硝酸铅均不能使其发生翅斑。Pb（NO_3）_2 ~（2-）能诱导突变点频率显著增加。此外，与γ射线单独处理相比，γ射线与PbCl 2或Pb（NO3）（2）的联合处理在记录的3类突变点频率上没有表现出显著的变化。这似乎表明，测试的先导化合物与电离辐射引起的遗传损伤的修复没有相互作用。当铅化合物在体内彗星试验中与血细胞进行评估时，Pb（NO3）（2）在诱导DNA损伤的显着增加中是有效的，具有直接的剂量反应模式。这些结果证实了血细胞彗星试验作为体内模型的有效性，并支持存在与铅暴露相关的遗传毒性风险的假设。(C)2011 Elsevier BM. All rights reserved.
The in vivo genotoxic activity of two inorganic lead compounds was studied in Drosophila melanogaster by measurement of two different genetic endpoints. We used the wing-spot test and the comet assay. The comet assay was conducted with larval haemocytes. The results from the wing-spot test showed that neither lead chloride, PbCl2, nor lead nitrate. Pb(NO3)(2), were able to induce significant increases in the frequency of mutant spots. In addition, the combined treatments with gamma-radiation and PbCl2 or Pb(NO3)(2) did not show significant variations in the frequency of the three categories of mutant spots recorded, compared with the frequency induced by gamma-radiation alone. This seems to indicate that the lead compounds tested do not interact with the repair of the genetic damage induced by ionizing radiation. When the lead compounds were evaluated in the in vivo comet assay with haemocytes, Pb(NO3)(2) was effective in inducing significant increases of DNA damage with a direct dose-response pattern. These results confirm the usefulness of the comet assay with haemocytes as an in vivo model and support the assumption that there is a genotoxic risk associated with lead exposure. (C) 2011 Elsevier BM. All rights reserved.