Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications
Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications
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DOI：
10.1016/j.biomaterials.2005.11.037
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发表时间：
2006-05-01
期刊：
影响因子：
14
通讯作者：
Lelkes, PI
中科院分区：
文献类型：
--
作者：
Li, MY;Guo, Y;Lelkes, PI
聚苯胺（PANi），一种导电聚合物，与天然蛋白质，明胶，共静电纺丝成纳米纤维，以研究这种共混物作为组织工程目的的导电支架的潜在应用。采用扫描电子显微镜（SEM）、电导率测量、力学拉伸测试和差示扫描量热法（DSC）对静电纺含PANi明胶纤维进行了表征。SEM分析的共混物纤维含有小于3%的PANi的总重量，揭示了均匀的纤维，没有相分离的证据，也证实了DSC。我们的数据表明，随着PANi的量的增加（从0到接近5%w/w），平均纤维尺寸从803 +/-121nm减小到61 +/-13nm（p < 0.01），拉伸模量从499 +/-207MPa增加到1384 +/-105MPa（p < 0.05）。DSC研究的结果进一步加强了我们的概念，即明胶与几个%的PAM掺杂导致明胶的物理化学性质的改变。为了测试PANi-明胶共混物作为支持细胞生长的纤维基质的有用性，将H9 c2大鼠心肌成肌细胞在纤维涂覆的玻璃盖玻片上培养。在细胞增殖和形态学方面评价细胞培养物。我们的研究结果表明，所有PANi-明胶共混物纤维支持H9 c2细胞附着和增殖到与对照组织培养处理的塑料（TCP）和光滑玻璃基板相似的程度。根据PANi的浓度，细胞最初在纤维基质上显示出不同的形态，但在1周后，所有培养物达到相似密度和形态的汇合。总之，这些结果表明，聚苯胺-明胶共混物纳米纤维可能提供一种新的导电材料，非常适合作为生物相容性支架的组织工程。(c)2005爱思唯尔有限公司保留所有权利。
Polyanifine (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi-contained gelatin fibers were characterized using scanning electron microscopy (SEM), electrical conductivity measurement, mechanical tensile testing, and differential scanning calorimetry (DSC). SEM analysis of the blend fibers containing less than 3% PANi in total weight, revealed uniform fibers with no evidence for phase segregation, as also confirmed by DSC. Our data indicate that with increasing the amount of PANi (from 0 to similar to 5% w/w), the average fiber size was reduced from 803 +/- 121 nm to 61 +/- 13 nm (p < 0.01) and the tensile modulus increased from 499 +/- 207 MPa to 1384 +/- 105 MPa (p < 0.05). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PAM leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1 week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering. (c) 2005 Elsevier Ltd. All rights reserved.