Gas chromatographic analysis of histamine in mahi-mahi (Coryphaena hippurus)
Gas chromatographic analysis of histamine in mahi-mahi (Coryphaena hippurus)
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DOI：
10.1021/jf020148x
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发表时间：
2002-08-14
影响因子：
6.1
通讯作者：
Marshall, MR
中科院分区：
文献类型：
--
作者：
Antoine, FR;Wei, CI;Marshall, MR
一些作者使用气相色谱 (GC) 作为定量工具研究了组胺，但所使用的方法并不总是适合，具体取决于食物的种类。经常提到的问题包括组胺从柱中洗脱不完全和峰拖尾。组胺之所以引起人们的兴趣，是因为它是所有鲭鱼中毒病例的共同因素，具有生理和生物活性，并且是鱼品质的化学指标。在这项研究中，采用改进的气相色谱法来定量鲯鳅 (Coryphaena hippurus) 中的组胺。 GC 方法的平均回收率为 67%，而 AOAC 荧光法的平均回收率为 90%。 GC 组胺值与 AOAC 荧光法的组胺值相关性为 0.96。温度程序、不分流/分流进样和分析物净化对于 GC 特性至关重要。组胺保留时间为 8.2 分钟。该方法允许使用峰高来定量和同时分析尸胺和腐胺。
Several authors have studied histamine using gas chromatography (GC) as a tool for quantitation, but the methods used were not always suitable depending on the kind of food. Problems frequently cited include incomplete histamine elution from the columns and peak tailing. Histamine is of interest because it is the factor common to all cases of scombroid poisoning, it has physiological and biological activity, and it is a chemical indicator of fish quality. In this study a modified GC method was used to quantify histamine in mahi-mahi (Coryphaena hippurus). Mean recovery was 67% for the GC method, compared with 90% for the AOAC fluorometric method. There was a 0.96 correlation of the GC histamine values with those of the AOAC fluorometric method. A temperature program, splitless/split injection, and analyte cleanup were essential for GC properties. Histamine retention time was 8.2 min. The method allowed peak height to be used for quantitation and simultaneous analysis of cadaverine and putrescine.