An Individual Nanocube-Based Plasmonic Biosensor for Real-Time Monitoring the Structural Switch of the Telomeric G-Quadruplex
An Individual Nanocube-Based Plasmonic Biosensor for Real-Time Monitoring the Structural Switch of the Telomeric G-Quadruplex
复制标题
一种基于纳米立方体的等离子体生物传感器，用于实时监测端粒 G-四链体的结构开关
作者：
Tian YY;Zhang L;Shen JJ;Wu LZ;He HZ;Ma DL;Leung CH;Wu WB;Fan QL;Huang W;Wang LH
在局域表面等离子体共振纳米技术的推动下，人们提出了一种在单个Au@Ag核壳纳米立方体（Au@Ag NC）上的简单而灵敏的等离子体适体纳米传感器（nanoaptasensor），用于实时监测G四链体结构的形成过程和钾离子（K+）的无标记分析。特别是，热力学参数分析表明，端粒DNA序列的顺序折叠过程中存在两种类型的结合状态，并伴随着自由能（ΔG）的显着变化。这种纳米适体传感器在监测 G-四链体结构转换的动态过程方面具有广阔的应用前景。
Promoted by the localized surface plasmon resonance nanotechnology, a simple and sensitive plasmonic aptamer nanosensor (nanoaptasensor) on an individual Au@Ag core‐shell nanocube (Au@Ag NC) has been proposed for real‐time monitoring of the formation process of G‐quadruplex structures and label‐free analysis of potassium ions (K+). In particular, the analysis of the thermodynamic parameters indicates that there are two types of binding states accompanied with a remarkable change of free energy (ΔG) in the sequential folding process of telomere DNA sequence. This nanoaptasensor has raised promising applications in monitoring the dynamic process of the structural switch of the G‐quadruplex.