Transcriptome analysis reveals thatinsulin is an immunomodulatory hormone in common carp
Transcriptome analysis reveals thatinsulin is an immunomodulatory hormone in common carp
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转录组分析表明胰岛素是鲤鱼的一种免疫调节激素
DOI：
10.1016/j.fsi.2016.10.018
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发表时间：
2016
影响因子：
4.7
通讯作者：
Fan Yu
中科院分区：
文献类型：
--
作者：
Yi Zhou;Wenjuan Yu;Huan Zhong;Jianlin Li;Hongxia Li;Feng He;Jie Zhou;Yongkai Tang;Juhua Yu;Fan Yu
鲤鱼（Cyprinus carpio）是我国及东亚地区广泛分布的淡水鱼类，也是重要的经济鱼类。最近的研究表明，胰岛素可以改变免疫基因的表达，因此，可以被认为是一种免疫调节激素。为了了解对胰岛素的免疫应答机制，我们使用注射胰岛素（5 μg/g体重）或盐水作为对照的鲤鱼肝脏进行了比较RNA-seq转录组分析。在过滤低质量读段并去除衔接子后，将干净的原始读段组装成60，421个单基因，平均长度为746.81 bp。此外，在公共数据库中进行blast搜索后，基于同源性注释了37，107个unigenes。采用每千字节的片段数法和EdgeR软件对差异表达基因进行鉴定。共发现782条差异表达基因，其中胰岛素注射组上调基因444条，下调基因338条。Gene Ontology分析表明，这些基因集中在葡萄糖代谢、激素分泌和免疫系统过程中.此外，153个富集的KEGG通路与差异表达的基因相关，包括Toll样受体（TLR）和核因子κ β（NF-κB）信号通路。注射胰岛素后，信号转导和转录激活因子1（10.56倍）、TLR 3（0.089倍）、激活蛋白-1（0.007倍）、肿瘤坏死因子-α（0.139倍）和巨噬细胞炎性蛋白-1 β（0.038倍）的表达均发生显著变化。本研究首次揭示了鲤鱼肝脏中胰岛素诱导的基因表达谱特征，为深入了解胰岛素作为免疫调节激素的分子机制提供了新的思路。
Common carp (Cyprinus carpio) is a widespread freshwater fish and economically important species in China and other East Asian countries. Recent studies suggest that insulin can alter the expression of immune genes and, thus, can be regarded as an immunomodulatory hormone. To understand the mechanism of the immune response to insulin, we performed a comparative RNA-seq transcriptome analysis using livers from common carp injected with insulin (5 μg/g bodyweight) or saline as a control. After filtering the low-quality reads and removing the adaptors, the clean raw reads were assembled into 60,421 unigenes with mean length of 746.81 bp. Furthermore, 37,107 unigenes were annotated based on homology after blast search in public databases. Differentially expressed genes were identified using the fragments per kb per million fragments method and EdgeR software. In total, 782 differentially expressed genes were found. Thereinto, 444 and 338 genes were upregulated and downregulated, respectively, in the insulin-injected group. A Gene Ontology analysis indicated that these genes were concentrated in glucose metabolism, hormone secretion, andimmune system processes. Moreover, 153 enriched KEGG pathways were associated with the differentially expressed genes, including the Toll-like receptor (TLR) and nuclear factor kappa beta (NF-κB) signaling pathways. Signal transducer and activator of transcription 1 (10.56-fold), TLR3 (0.089-fold), activator protein-1 (0.007-fold), tumor necrosis factor-α (0.139-fold), and macrophage inflammatory protein-1β (0.038-fold) expression were significantly changed after the insulin injection. This study characterized the profile of genes expression response to insulin in common carp liver for the first time and provided new insight into understanding the molecular mechanism of insulin as an immunomodulatory hormone.