Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota
Lactobacillus fermentum species ameliorate dextran sulfate sodium-induced colitis by regulating the immune response and altering gut microbiota
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DOI：
10.1080/19490976.2019.1589281
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发表时间：
2019-11-02
期刊：
影响因子：
12.2
通讯作者：
Ko, Gwangpyo
中科院分区：
文献类型：
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作者：
Jang, You Jin;Kim, Woon-Ki;Ko, Gwangpyo
我们评估了从健康韩国人粪便中分离的新型发酵乳杆菌菌株（KBL 374和KBL 375）的免疫代谢功能。在用L.发酵菌株KBL 374或KBL 375菌株。当这些菌株口服给予葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠时，两种L。发酵菌株KBL 374和KBL 375对体重、疾病活动指数评分、结肠长度、盲肠重量和组织学评分显示有益效果。此外，L.发酵菌KBL 374和KBL 375通过改善肠屏障功能和减少白细胞浸润来调节先天性免疫应答。与PBMC数据一致，L.与DSS对照小鼠相比，发酵KBL 374和KBL 375处理的DSS小鼠显示结肠中Th 1-、Th 2-和Th 17-相关细胞因子水平降低，IL-10增加。给药L.发酵菌KBL 374或KBL 375对小鼠的作用增加了肠系膜淋巴结中的⑶ 4 + ⑶ 25 + Foxp 3 +Treg细胞群体。此外，L.施用发酵菌KBL 374或KBL 375重塑并增加了肠道微生物群的多样性。特别是L.发酵菌KBL 375增加了有益微生物如乳杆菌属的丰度。和阿克曼氏菌属（Akkermansia spp.）两个湖发酵菌KBL 374和KBL 375可以通过调节免疫应答和改变肠道微生物群的组成来减轻肠道中的炎性疾病，例如炎性肠病。
We evaluated immunometabolic functions of novel Lactobacillus fermentum strains (KBL374 and KBL375) isolated from feces of healthy Koreans. The levels of inflammatory cytokines, such as interleukin (IL)-2, interferon-gamma, IL-4, IL-13, and IL-17A, were decreased, and that of the anti-inflammatory cytokine IL-10 was increased, in human peripheral blood mononuclear cells (PBMCs) treated with the L. fermentum KBL374 or KBL375 strain. When these strains were orally administered to mice with dextran sulfate sodium (DSS)-induced colitis, both L. fermentum KBL374 and KBL375 showed beneficial effects on body weight, disease activity index score, colon length, cecal weight, and histological scores. Furthermore, both L. fermentum KBL374 and KBL375 modulated the innate immune response by improving gut barrier function and reducing leukocyte infiltration. Consistent with the PBMC data, both L. fermentum KBL374- and KBL375-treated DSS mice demonstrated decreased Th1-, Th2-, and Th17-related cytokine levels and increased IL-10 in the colon compared with the DSS control mice. Administration of L. fermentum KBL374 or KBL375 to mice increased the CD4+CD25+Foxp3+Treg cell population in mesenteric lymph nodes. Additionally, L. fermentum KBL374 or KBL375 administration reshaped and increased the diversity of the gut microbiota. In particular, L. fermentum KBL375 increased the abundance of beneficial microorganisms, such as Lactobacillus spp. and Akkermansia spp. Both L. fermentum KBL374 and KBL375 may alleviate inflammatory diseases, such as inflammatory bowel disease, in the gut by regulating immune responses and altering the composition of gut microbiota.