PMMA/PDMS valves and pumps for disposable microfluidics
PMMA/PDMS valves and pumps for disposable microfluidics
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用于一次性微流体的 PMMA/PDMS 阀门和泵
DOI：
10.1039/b907254c
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Yang, Chaoyong James
中科院分区：
文献类型：
--
作者：
Zhang, Wenhua;Lin, Shuichao;Yang, Chaoyong James
聚甲基丙烯酸甲酯(PMMA)因其成本低、光学透明性好、机械/化学性能好、制备工艺简单等优点，在微流控器件中越来越受到人们的青睐。它已被用于制造微混合器、聚合酶链式反应反应器、毛细管电泳法和许多其他微器件。本文介绍了基于PMMA的气动微型阀和微型泵的设计、制造、表征和应用。阀门和泵是通过在PMMA流体通道和歧管晶片之间夹一层PDMS膜来制造的。阀门的关闭或打开可以通过计算机调节的电磁阀调节气动层上排气室中的压力来控制。该阀门在60kpa流体压力下可提供高达15.4MU的L S(-1)压力，并能可靠地密封高达60kpa的向前流体压力。PMMA隔膜泵只需串联三个阀门即可组装。通过改变阀门的体积或开启时间，可以精确地实现每秒从NL到毫升的泵送速度。基于PMMA的阀门和泵在一次性自动核酸提取微芯片中进行了进一步测试，以从人类全血中提取DNA。DNA提取效率约为25%，提取DNA的260 nm/280 nm紫外吸收比为1.72。由于PMMA阀和泵具有价格低廉、制造简单、坚固耐用和易于集成等优点，将在便携式和一次性微流控设备中得到广泛的应用。
Poly(methyl methacrylate) (PMMA) is gaining in popularity in microfluidic devices because of its low cost, excellent optical transparency, attractive mechanical/chemical properties, and simple fabrication procedures. It has been used to fabricate micromixers, PCR reactors, CE and many other microdevices. Here we present the design, fabrication, characterization and application of pneumatic microvalves and micropumps based on PMMA. Valves and pumps are fabricated by sandwiching a PDMS membrane between PMMA fluidic channel and manifold wafers. Valve closing or opening can be controlled by adjusting the pressure in a displacement chamber on the pneumatic layer via a computer regulated solenoid. The valve provides up to 15.4 mu L s(-1) at 60 kPa fluid pressure and seals reliably against forward fluid pressure as high as 60 kPa. A PMMA diaphragm pump can be assembled by simply connecting three valves in series. By varying valve volume or opening time, pumping rates ranging from nL to mL per second can be accurately achieved. The PMMA based valves and pumps were further tested in a disposable automatic nucleic acid extraction microchip to extract DNA from human whole blood. The DNA extraction efficiency was about 25% and the 260 nm/280 nm UV absorption ratio for extracted DNA was 1.72. Because of its advantages of inexpensive, facile fabrication, robust and easy integration, the PMMA valve and pump will find their wide application for fluidic manipulation in portable and disposable microfluidic devices.