mRNA-miRNA-Seq Reveals Neuro-Cardio Mechanisms of Crude Oil Toxicity in Red Drum ( Sciaenops ocellatus).
mRNA-miRNA-Seq Reveals Neuro-Cardio Mechanisms of Crude Oil Toxicity in Red Drum ( Sciaenops ocellatus).
复制标题
DOI：
10.1021/acs.est.9b00150
复制
发表时间：
2019-02
影响因子：
11.4
通讯作者：
E. G. Xu;A. J. Khursigara;Shuying Li;A. Esbaugh;S. Dasgupta;D. Volz;D. Schlenk
中科院分区：
文献类型：
--
作者：
E. G. Xu;A. J. Khursigara;Shuying Li;A. Esbaugh;S. Dasgupta;D. Volz;D. Schlenk
原油中存在的多环芳烃（PAHs）可导致鱼类的基因失调和发育障碍。然而，改变基因调控的机制还不清楚。在这项研究中，红石首鱼（Sciaenops ocellatus）的幼虫暴露于深水地平线（DWH）溢油源油（6.8，13.7和35.9 μg/L的总多环芳烃）和风化浮油（4.7，8.1和18.0 μg/L的总多环芳烃）的水容纳部分。通过下一代测序和深入的生物信息学分析，探索了与DWH油毒性相关的全球mRNA-microRNA功能网络。在所有暴露浓度下，源油和浮油均显著改变了miR-18 a、miR-27 b和miR-203 a的表达。与观察到的浓度依赖性形态学变化一致，这些microRNA的靶mRNA主要参与神经心脏系统发育过程和相关的关键信号传导途径，如轴突导向信号传导、神经元中的cAMP反应元件结合蛋白信号传导、钙信号传导和心脏肥大中的核因子活化T细胞信号传导。结果表明，原油的发育毒性可能是由于microRNA和相关靶基因的异常表达造成的，尤其是对神经系统。此外，我们还提供了一个利用非模型物种的mRNA-microRNA-seq数据进行系统毒性评价的案例研究。
Polycyclic aromatic hydrocarbons (PAHs) present in crude oil can cause global gene dysregulation and developmental impairment in fish. However, the mechanisms that alter gene regulation are not well understood. In this study, larval red drum ( Sciaenops ocellatus) were exposed to water accommodated fractions of source oil (6.8, 13.7, and 35.9 μg/L total PAHs) and weathered slick oil (4.7, 8.1, and 18.0 μg/L total PAHs) from the Deepwater Horizon (DWH) oil spill. The global mRNA-microRNA functional networks associated with the toxicity of DWH oil were explored by next-generation sequencing and in-depth bioinformatics analyses. Both source and slick oil significantly altered the expression of miR-18a, miR-27b, and miR-203a across all exposure concentrations. Consistent with the observed concentration-dependent morphological changes, the target mRNAs of these microRNAs were predominantly involved in neuro-cardio system development processes and associated key signaling pathways such as axonal guidance signaling, cAMP-response-element-binding protein signaling in neurons, calcium signaling, and nuclear-factor-of-activated T cells signaling in cardiac hypertrophy. The results indicated that the developmental toxicity of crude oil may result from the abnormal expression of microRNAs and associated target genes, especially for the nervous system. Moreover, we provide a case study for systematic toxicity evaluation leveraging mRNA-microRNA-seq data using nonmodel species.