Development of a monoclonal antibody to detect predation of the brown planthopper Nilaparvata lugens (Stål)
Development of a monoclonal antibody to detect predation of the brown planthopper Nilaparvata lugens (Stål)
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DOI：
10.1080/09670870400000358
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发表时间：
2004-10
影响因子：
1.5
通讯作者：
Weichun Zhao;Jia-an Cheng;Zheng-Xian Chen
中科院分区：
文献类型：
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作者：
Weichun Zhao;Jia-an Cheng;Zheng-Xian Chen
使用杂交瘤技术，针对褐飞虱 Nilaparvata lugens (Stål) 的全匀浆开发了单克隆抗体 (McAb) 4B8。它与 BPH 的所有阶段发生反应，但不与通过间接酶联免疫吸附测定 (ELISA) 测试的其他昆虫和捕食者物种发生交叉反应。免疫扩散显示其属于 IgG3 亚类。 4B8细胞系产生的腹水具有高吸光度值，直到通过间接ELISA测试将其稀释超过6.5536×107倍。在 25°C 下，一只雌性被拟环豹蛛吞食约 2.13 天后，它可以识别 BPH 蛋白。延长的抗原检测周期和抗体的高特异性结果表明4B8可用于研究飞虱与其捕食者之间的田间相互作用。应用单克隆抗体评估亚热带水稻生态系统中四种主要蜘蛛的相对重要性，结果表明，P.pseudoannulata 比 Ummeliata 昆虫更重要，而后者又比 Tetragnatha sp 更重要。和八斑鞘体虫。
A monoclonal antibody (McAb), 4B8, was developed to whole homogenates of the brown planthopper, Nilaparvata lugens (Stål), using hybridoma technology. It reacted with all the stages of BPH but did not cross-react with other insects and predator species tested with an indirect enzyme-linked immunosorbent assay (ELISA). Immunodiffusion revealed it to belong to the IgG3 subclass. The ascites developed with 4B8 cell lines had high absorbance values until it was diluted over 6.5536 × 107 times tested by indirect ELISA. It could recognize the BPH proteins after one female was ingested by Pardosa pseudoannulata for about 2.13 days at 25°C. The results of extended antigen detection period and the high specificity of the antibody indicated that 4B8 could be used to study interactions between planthoppers and their predators in the field. Application of the McAb to assess relative importance of four major spider species in subtropical rice ecosystem showed that P. pseudoannulata was more important than Ummeliata insecticeps, and those were more important than Tetragnatha sp. and Coleosoma octomaculatum.