A MatP-divisome interaction coordinates chromosome segregation with cell division in E. coli
A MatP-divisome interaction coordinates chromosome segregation with cell division in E. coli
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DOI：
10.1038/emboj.2012.128
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发表时间：
2012-07-18
期刊：
影响因子：
11.4
通讯作者：
Boccard, Frederic
中科院分区：
文献类型：
--
作者：
Espeli, Olivier;Borne, Romain;Boccard, Frederic
细菌中染色体分离的起始是通过在复制起点附近起作用的蛋白质实现的。在这里，我们报告说，大肠杆菌染色体的末端区域的精确编排也受到严格控制。末端（Ter）宏结构域（MD）的分离涉及结构因子MatP。我们的特点是，迁移的Ter MD从新的极中细胞和其随后的持久定位在中细胞依赖于几个过程。首先，Ter DNA的复制是伴随着它从新极到中间细胞的招募，其顺序与遗传图谱上的位置相关。第二，使用一个菌株携带线性染色体的Ter MD分裂成两部分，我们表明，复制体被重新定位在中间细胞时，复制的Ter发生。第三，我们证明，锚定的Ter MD中细胞依赖于特定的相互作用的MatP与分裂器相关的蛋白质ZapB。我们的研究结果揭示了如何隔离的Ter MD是集成在细胞周期控制。The EMBO Journal（2012）31，3198-3211. doi：10.1038/daj.2012.128; 2012年5月11日在线发布
Initiation of chromosome segregation in bacteria is achieved by proteins acting near the origin of replication. Here, we report that the precise choreography of the terminus region of the Escherichia coli chromosome is also tightly controlled. The segregation of the terminus (Ter) macrodomain (MD) involves the structuring factor MatP. We characterized that migration of the Ter MD from the new pole to mid-cell and its subsequent persistent localization at mid-cell relies on several processes. First, the replication of the Ter DNA is concomitant with its recruitment from the new pole to mid-cell in a sequential order correlated with the position on the genetic map. Second, using a strain carrying a linear chromosome with the Ter MD split in two parts, we show that replisomes are repositioned at mid-cell when replication of the Ter occurs. Third, we demonstrate that anchoring the Ter MD at mid-cell depends on the specific interaction of MatP with the division apparatus-associated protein ZapB. Our results reveal how segregation of the Ter MD is integrated in the cell-cycle control. The EMBO Journal (2012) 31, 3198-3211. doi:10.1038/emboj.2012.128; Published online 11 May 2012