Effects of long-term sample preservation on flow cytometric analysis of natural populations of pico-and nanophytoplankton
Effects of long-term sample preservation on flow cytometric analysis of natural populations of pico-and nanophytoplankton
复制标题
DOI：
10.1007/s10872-006-0107-x
复制
发表时间：
2006-12
影响因子：
2.3
通讯作者：
Mitsuhide Sato;S. Takeda;K. Furuya
中科院分区：
文献类型：
--
作者：
Mitsuhide Sato;S. Takeda;K. Furuya
研究了长期保存对自然海洋微微型浮游植物种群流式细胞仪参数的影响。根据Vaulotet等人的说法，在北太平洋贫营养亚热带和亚北极地区收集的种群用戊二醛固定并冷冻在液氮中。（1989年）。在6个月的贮藏过程中，叶绿素红荧光在所有检测组中都有所下降，而前向光散射在聚球藻和原绿球藻中增强，而在纳米真核生物中减弱。除聚球藻外，其余细胞损失均不显著。当分析储存超过一个月的样本的流式细胞术数据时，需要谨慎。
The effects of long-term preservation on flow cytometric parameters of natural oceanic populations of pico-and nanophytoplankton have been examined. Populations collected at oligotrophic subtropical and subarctic locations in the North Pacific were fixed with glutaraldehyde and frozen in liquid nitrogen, according to Vaulotet al.(1989). During six months’ storage, chlorophyll red fluorescence declined in all the groups examined, while forward light scatter was enhanced inSynechococcusandProchlorococcus, and weakened in nanoeucaryotes. Cell loss was not significant except forSynechococcus. Caution is required when analyzing flow cytometric data of samples stored for more than a month.