Magnetic nanoparticles: Inner ear targeted molecule delivery and middle ear implant
Magnetic nanoparticles: Inner ear targeted molecule delivery and middle ear implant
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DOI：
10.1159/000090685
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发表时间：
2006-01-01
影响因子：
1.6
通讯作者：
Dormer, KJ
中科院分区：
文献类型：
--
作者：
Kopke, RD;Wassel, RA;Dormer, KJ
初步研究了由磁铁矿（Fe3O4）组成的超顺磁性氧化铁纳米颗粒（SNP）作为治疗性分子传递到内耳的载体，以及作为能够产生与听觉功能相关的生物力学力的中耳植入物。磁铁矿SNP的合成，然后包被二氧化硅或聚（D，L，-丙交酯-共乙二醇酯）或在油酸或葡聚糖包被下获得。在几种圆窗膜模型（体外细胞培养、体内大鼠和豚鼠以及人颞骨）中，永久磁场产生的力足以将它们拉过组织或嵌入中耳上皮。通过光镜、电镜、细胞培养动力学和器官型细胞培养毛细胞存活率研究了生物相容性，未发现可测量的毒性。在中耳植入SNP的豚鼠身上施加正弦磁场，导致中耳位移达到90 dB SPL。版权所有(C) 2006 S. Karger AG，巴塞尔
Superparamagnetic iron oxide nanoparticles ( SNP) composed of magnetite (Fe3O4) were studied preliminarily as vehicles for therapeutic molecule delivery to the inner ear and as a middle ear implant capable of producing biomechanically relevant forces for auditory function. Magnetite SNP were synthesized, then encapsulated in either silica or poly (D,L,-Lactide-co-glycolide) or obtained commercially with coatings of oleic acid or dextran. Permanent magnetic fields generated forces sufficient to pull them across tissue in several round window membrane models ( in vitro cell culture, in vivo rat and guinea pig, and human temporal bone) or to embed them in middle ear epithelia. Biocompatibility was investigated by light and electron microscopy, cell culture kinetics, and hair cell survival in organotypic cell culture and no measurable toxicity was found. A sinusoidal magnetic field applied to guinea pigs with SNP implanted in the middle ear resulted in displacements of the middle ear comparable to 90 dB SPL. Copyright (C) 2006 S. Karger AG, Basel.