STUDIES OF ACUTE PHASE PROTEIN .1. AN IMMUNOHISTOCHEMICAL METHOD FOR LOCALIZATION OF CX-REACTIVE PROTEIN IN RABBITS - ASSOCIATION WITH NECROSIS IN LOCAL INFLAMMATORY LESIONS
STUDIES OF ACUTE PHASE PROTEIN .1. AN IMMUNOHISTOCHEMICAL METHOD FOR LOCALIZATION OF CX-REACTIVE PROTEIN IN RABBITS - ASSOCIATION WITH NECROSIS IN LOCAL INFLAMMATORY LESIONS
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DOI：
10.1084/jem.114.6.961
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发表时间：
1961-01-01
影响因子：
15.3
通讯作者：
KAPLAN, MH
中科院分区：
文献类型：
--
作者：
KUSHNER, I;KAPLAN, MH
本文提出了一种兔体内cx反应蛋白的免疫组织化学定位方法，该方法基于使用一种明确的抗血清和该抗原所需的严格固定技术。在肌内注射甲状腺疫苗诱导炎症病变和CxRP反应的动物中，cx反应蛋白仅局限于肌纤维局部炎症区域，表现出坏死改变的形态学证据。在这些改变的纤维中，在肌纤维的外周节段或肌上皮下肌浆、肌浆中的分散沉积物和液泡包涵体中观察到CxRP。除了在血管管腔或间质中发现外，任何时候在炎性病变的多核细胞或单核细胞中都没有发现CxRP，也没有在对侧肌肉、区域或远端淋巴结、肝脏、脾脏、胸腺、心脏或肾脏中发现。在注射炎症刺激后8至10小时的潜伏期后，首先在炎症部位的坏死肌纤维中检测到CxRP，并且在48小时的实验中可以在这些部位证明。在血液中出现之前，在炎症部位无法观察到。用氮芥诱导粒细胞减少后，发现坏死肌纤维在局部病变部位有相同的组织学定位。这些发现与假设相一致，即CxRP是在炎症部位局部形成的，由经历坏死变化的组织成分形成。另外，不能排除炎症部位血液的继发性沉积，但鉴于未能获得其他器官中CxRP细胞定位的证据，这种可能性较小。
A method is presented for the immunohistochemical localization of Cx-reactive protein in rabbits, based on the use of a defined anti-serum and rigorous fixation techniques requisite for this antigen. In animals in which inflammatory lesions and CxRP response were induced by intramuscular injection of thyroid vaccine, Cx-reactive protein was localized only in the area of local inflammation within muscle fibers showing morphologic evidence of necrotic change. Within such altered fibers, CxRP was observed in peripheral segments of myofiber or in subsarcolemmal sarcoplasm, in scattered deposits in sarcoplasm, and in vacuolar inclusions. No CxRP was found at any time in polymorpho-nuclear or mononuclear cells in the inflammatory lesion, nor in con-tralateral muscle, regional or distal lymph nodes, liver, spleen, thymus, heart, or kidney, except as traces in lumens of vessels or interstitium. CxRP was first detected in necrotic myofibers at the inflammatory site after a latent period of 8 to 10 hours following injection of the inflammatory stimulus and could be demonstrated in these sites for the 48 hours of the experiment. It could not be observed at the inflammatory site before appearance in the blood. Identical histologic localization in necrotic myofibers at the site of the local lesions was found following induction of granulocytopenia with nitrogen mustard. These findings are consistent with the hypothesis that CxRP is formed locally at the site of inflammation from tissue elements undergoing necrotic change. Alternatively, secondary deposition from the blood at the inflammatory site cannot be excluded, but is considered less likely in view of the failure to obtain evidence of a cellular localization of CxRP in other organs.