Alteration of immune functions and Th1/Th2 cytokine balance in nicotine-induced murine macrophages: Immunomodulatory role of eugenol and N-acetylcysteine
Alteration of immune functions and Th1/Th2 cytokine balance in nicotine-induced murine macrophages: Immunomodulatory role of eugenol and N-acetylcysteine
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DOI：
10.1016/j.intimp.2010.12.020
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发表时间：
2011-04-01
影响因子：
5.6
通讯作者：
Roy, Somenath
中科院分区：
文献类型：
--
作者：
Mahapatra, Santanu Kar;Bhattacharjee, Surajit;Roy, Somenath
The aim of this study was to evaluate the immune functions by nicotine-induced murine peritoneal macrophages, and Th1/Th2 cytokine balance in it, and concurrently to establish the immunomodulatory role of eugenol, and N-acetylcysteine in nicotine-induced macrophages. Eugenol was isolated from Ocimum gratissimum, and characterized by HPLC, FTIR, and H-1 NMR. The cytotoxic effect of isolated eugenol was studied in murine peritoneal macrophages at various concentrations (0.1-50 mu g/ml) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. To evaluate the immunomodulatory role of eugenol and N-acetylcysteine, ROS and nitrite generations, phenotype functions by macrophages were studied. The effect of eugenol and N-acetylcysteine on the release of Th1 cytokines (TNF-alpha., IL-12) and Th2 cytokines (IL-10, TGF-beta) was measured by ELISA, and the expression of these cytokines at mRNA level were analyzed by real-time PCR. Eugenol, at a dose of 15 mu g/ml, showed less cytotoxiciti to the macrophages and it significantly reduced the nicotine-induced ROS, NO generation, and iNOSII expression. Similar kinds of response were observed in the presence of N-acetylcysteine (1 mu g/ml). We have found the decreased adherence, chemotaxis, phagocytosis and intracellular killing of bacteria in nicotine treated macrophages, whereas eugenol and N-acetylcysteine with nicotine treatment enhanced these cellular functions by macrophages significantly ( p
