A(H1N1)pdm09 Influenza Viruses Replicating in Ferret Upper or Lower Respiratory Tract Differed in Onward Transmission Potential by Air
A(H1N1)pdm09 Influenza Viruses Replicating in Ferret Upper or Lower Respiratory Tract Differed in Onward Transmission Potential by Air
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DOI：
10.1093/infdis/jiab286
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发表时间：
2021-05-24
影响因子：
6.4
通讯作者：
Yen, Hui-Ling
中科院分区：
文献类型：
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作者：
Xie, Chenyi;Su, Wen;Yen, Hui-Ling
背景。A(HINI)pdm09流感病毒在呼吸道上皮细胞中有效复制，并通过受感染宿主排出的飞沫和气溶胶传播。流感病毒在上呼吸道和下呼吸道上皮细胞中复制的相对传播潜力尚未完全确定。将每个基因片段有2个同义突变的野生型和条形码A(HINI)pdm09病毒通过鼻内和气管内途径接种到雪貂体内。接种后第2天，天真受者暴露于接种供体呼出的气体中8小时。通过下一代测序监测野生型和条形码基因型的传播潜力。可传播的空气颗粒从供体雪貂的上呼吸道而不是下呼吸道上皮细胞中呼吸出来。在上呼吸道和下呼吸道组织中复制的病毒种群混合有限。雪貂上呼吸道上皮被定位为产生携带流感病毒的颗粒并通过空气传播的解剖部位。我们的研究结果表明，疫苗和抗病毒药物应旨在减少上呼吸道的病毒载量，以预防流感传播。
Background. A(HINI)pdm09 influenza viruses replicate efficiently in respiratory epithelia and are transmitted via respiratory droplets and aerosols expelled by infected hosts. The relative onward transmission potential of influenza viruses replicating in the upper and lower respiratory epithelial cells has not been fully defined.Methods. Wild-type and barcoded A(HINI)pdm09 viruses that differed by 2 synonymous mutations per gene segment were inoculated into ferrets via intranasal and intratracheal routes. Naive recipients were exposed to the exhaled breath of inoculated donors for 8 hours on day 2 postinoculation. Onward transmission potential of wild-type and barcoded genotypes were monitored by next generation sequencing.Results. Transmissible airborne particles were respired from the upper but not the lower respiratory epithelial cells of donor ferrets. There was limited mixing of viral populations replicating in the upper and lower respiratory tissues.Conclusions. The ferret upper respiratory epithelium was mapped as the anatomic site that generated influenza virus-laden particles mediating onward transmission by air. Our results suggest that vaccines and antivirals should aim to reduce viral loads in the upper respiratory tract for prevention of influenza transmission.