Determination of metanephrines in plasma by liquid chromatography with electrochemical detection.
Determination of metanephrines in plasma by liquid chromatography with electrochemical detection.
复制标题
采用电化学检测液相色谱法测定血浆中的变肾上腺素。
DOI：
10.1093/clinchem/39.1.97
复制
发表时间：
1993
影响因子：
9.3
通讯作者：
I. Kopin
中科院分区：
文献类型：
--
作者：
J. Lenders;G. Eisenhofer;I. Armando;H. Keiser;D. Goldstein;I. Kopin
肾上腺素是儿茶酚胺的O-甲基化代谢物。我们报告了使用液相色谱电化学检测器来测定血浆中的去甲甲肾上腺素（NMN）和间甲肾上腺素（MN）的浓度。本文对32例正常人和23例嗜铬细胞瘤患者血浆NMN和MN浓度进行了比较。将血浆中的异肾上腺素吸附到阳离子交换柱上，用氨性甲醇洗脱。将干燥的残余物溶于移动的相中，并进样至反相柱上。NMN和MN从1 mL血浆中的回收率平均为50- 70%，结果在0.13-55 pmol范围内随进样量线性变化。NMN和MN的检测限分别为25 fmol和50 fmol。测定内CV <5%。在正常志愿者和住院患者中，NMN的血浆浓度范围为0.12至0.73 nmol/L（平均0.38 nmol/L），MN范围为0.06至0.63 nmol/L（平均0.19 nmol/L）。所有23例嗜铬细胞瘤患者的血浆NMN浓度均升高（范围1-172 nmol/L），而MN浓度仅在9例患者中升高（范围0.10-382 nmol/L）。该方法灵敏度高、可靠性好，为研究儿茶酚胺类物质的神经元代谢提供了一种新的方法。该方法也可用于嗜铬细胞瘤的诊断。
Metanephrines are O-methylated metabolites of catecholamines. We report the use of liquid chromatography with electrochemical detection to determine plasma concentrations of normetanephrine (NMN) and metanephrine (MN). Plasma NMN and MN in 32 normal volunteers and inpatients were compared with concentrations in 23 patients with pheochromocytoma. Metanephrines were adsorbed from plasma onto a cation-exchange column and eluted with ammoniacal methanol. The dried residue was dissolved in mobile phase and injected onto a reversed-phase column. Recoveries of NMN and MN from 1 mL of plasma averaged 50-70%, and results varied linearly with quantity injected over a range of 0.13-55 pmol. The detection limit was 25 fmol for NMN and 50 fmol for MN. Intra-assay CVs were < 5%. In normal volunteers and inpatients, plasma concentrations of NMN ranged between 0.12 and 0.73 nmol/L (mean 0.38 nmol/L), and MN between 0.06 and 0.63 nmol/L (mean 0.19 nmol/L). Plasma NMN concentrations were increased in all 23 patients with pheochromocytoma (range 1-172 nmol/L), whereas MN concentrations (range 0.10-382 nmol/L) were increased in only 9 patients. The assay method is reliable and sensitive and offers an approach to examine the extraneuronal metabolism of catecholamines. The method may also be useful in the diagnosis of pheochromocytoma.