Molecular inventory of faecal microflora in patients with Crohn's disease
Molecular inventory of faecal microflora in patients with Crohn's disease
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DOI：
10.1016/j.femsec.2004.05.005
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发表时间：
2004-10-01
影响因子：
4.2
通讯作者：
Doré, J
中科院分区：
文献类型：
--
作者：
Mangin, I;Bonnet, R;Doré, J
肠道微生物群落参与了克罗恩病的发病机制，但由于不可能培养出许多优势肠道细菌，对其潜在异常的了解一直受到限制。采用随机克隆的细菌16S核糖体DNA序列分析方法，比较了4例克罗林病患者和4例对照的粪便优势种。虽然在患者的粪便微生物区系中观察到了显著的个体间差异，但根据其操作分类单位组成，仍有三个患者与对照组有较远的亲缘关系。类杆菌、Vidgatus和密切相关的生物体是所有患者共有的唯一分子物种，并表现出异常高的发生率。仅在两例回结肠克罗恩病患者中分离到了大肠杆菌克隆。此外，许多克隆属于通常在健康人粪便微生物区系中不占优势的系统发育类群或物种：果胶菌属、苏特氏菌属、韦氏微生物属、梭杆菌属、Disporicum属、糖基梭状芽胞杆菌属、丛枝状梭状芽胞杆菌属、无菌梭状芽胞杆菌属和产气荚膜梭菌属。(C)2004年欧洲微生物学会联合会。爱思唯尔出版，版权所有。
Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. Using sequence analysis of randomly cloned bacterial 16S ribosomal DNA, the dominant faecal species from four Crolin's disease patients and four controls were compared. Whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly related to controls on the basis of their operational taxonomic unit composition. Bacteroides vidgatus and closely related organisms represented the only molecular species shared by all patients and exhibited an unusually high rate of occurrence. Escherichia coli clones were isolated only in two patients with ileocolonic Crohn's disease. Moreover, numerous clones belonged to phylogenetic groups or species that are commonly not dominant in the faecal microflora of healthy subjects: Pectinatus, Sutterella, Verritcomicrobium, Fusobacterium, Clostridium disporicum, clostridium glycolicum, Clostridium ramosum, Clostridium innocuum and Clostridium perfringens. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.