Dynamics of Microfilaments are Similar, but Distinct from Microtubules During Cytokinesis in Living, Dividing Plant-Cells
Dynamics of Microfilaments are Similar, but Distinct from Microtubules During Cytokinesis in Living, Dividing Plant-Cells
复制标题
DOI：
10.1002/cm.970240302
复制
发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Dahong Zhang;P. Wadsworth;P. K. Hepler
中科院分区：
文献类型：
--
作者：
Dahong Zhang;P. Wadsworth;P. K. Hepler
对紫露草属植物雄蕊毛细胞成膜体细胞骨架的发育和动态进行了研究。微管和肌动蛋白微丝已经通过显微注射羧基荧光素标记的脑微管蛋白或罗丹明鬼笔环肽来鉴定。检查与共聚焦激光扫描显微镜允许顺序成像的荧光细胞骨架元素在单个活细胞通过分裂的进展。成膜体微管最初通过预先存在的区带间微管的侧向聚结而出现。随着胞质分裂的进行，这些紧密聚集的微管长度缩短，并向细胞周边离心扩张。相比之下，成膜体微丝出现从头在后期与重建的子核的近端表面密切相关。微丝的方向平行于微管，但明显不占据赤道区的微管交错和细胞板囊泡聚集和融合。随着发育的进行，微丝的长度缩短，周长扩大，类似于微管，尽管它们仍然被排除在细胞板区域之外。在细胞板形成的终末阶段，微管首先在成膜体的中心区域降解，然后向边缘降解，而微丝在整个成膜体中更均匀地分解。
The development and dynamics of the phragmoplast cytoskeleton have been analyzed in living stamen hair cells of Tradescantia. Microtubules and actin microfilaments have been identified by microinjecting either carboxyfluorescein labeled brain tubulin or rhodamine phalloidin. Examination with the confocal laser scanning microscope has permitted sequential imaging of the fluorescent cytoskeletal elements in single living cells progressing through division. Phragmoplast microtubules initially emerge through the lateral coalescence of preexisting interzone microtubules. As cytokinesis progresses, these tightly clustered microtubules shorten in length and expand centrifugally toward the cell periphery. By contrast, the phragmoplast microfilaments appear to arise de novo in late anaphase in close association with the proximal surfaces of the reconstituting daughter nuclei. The microfilaments are oriented parallel to the microtubules but conspicuously do not occupy the equatorial region where microtubules interdigitate and where the cell plate vesicles aggregate and fuse. As development proceeds the microfilaments shorten in length and expand in girth, similar to microtubules, although they remain excluded from the cell plate region. In terminal phases of cell plate formation, microtubules degrade first in the central regions of the phragmoplast and later toward the edges, whereas microfilaments break down more uniformly throughout the phragmoplast.