At which surface roughness do claws cling? Investigations with larvae of the running water mayfly Epeorus assimilis (Heptageniidae, Ephemeroptera)
At which surface roughness do claws cling? Investigations with larvae of the running water mayfly Epeorus assimilis (Heptageniidae, Ephemeroptera)
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DOI：
10.1016/j.zool.2011.11.003
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发表时间：
2012-12-01
期刊：
影响因子：
2
通讯作者：
Koop, Jochen H. E.
中科院分区：
文献类型：
--
作者：
Ditsche-Kuru, Petra;Barthlott, Wilhelm;Koop, Jochen H. E.
跗爪是节肢动物中常见的附着装置，与基底表面的不规则性相关联。大多数昆虫有两个跗爪，但一些水生昆虫的幼虫，如蜉蝣目和毛翅目，在他们的跗上只有一个爪。表面粗糙度的范围，足以为功能的水生无脊椎动物的单爪尚未调查。在本研究中，我们调查了蜉蝣，Epeorus同化幼虫的爪的功能所需的表面纹理。我们进行了附件实验，使用重复定义的表面粗糙度，白色光轮廓，录像和扫描电子显微镜。结果表明，选择合适的粗糙度顺序是非常重要的。切割长度约为爪尖直径的30倍的设置被证明最适合描述与E.同化幼虫常见的技术粗糙度参数，如R-a，R-q或R-z提供了足够的差异之间的测试基层。E.同化幼虫需要最小的表面粗糙度（R-a类似于6.0 μ m），以便它们的爪子抓持。在较小的表面粗糙度，幼虫离开底层。这是令人惊讶的，因为E。已观察到同化幼虫甚至附着在光滑表面上。基于在本实验中使用无菌基质，我们假设覆盖生物膜相当大地影响幼虫的附着。(C)2012 Elsevier GmbH. All rights reserved.
Tarsal claws, which are common attachment devices in arthropods, interlock with the surface irregularities of the substrata. Most insects have two tarsal claws, but the larvae of some aquatic insects, such as Ephemeroptera and Trichoptera, bear only one claw on their tarsi. The range of surface roughness that is sufficient for the function of the single claws of aquatic invertebrates has not yet been investigated. In the present study, we investigated the surface texture required for the function of the claws of larvae of the mayfly, Epeorus assimilis. We conducted attachment experiments using replications with defined surface roughness, white light profilometry, videotaping and scanning electron microscopy. The results demonstrate that the choice of an appropriate roughness order is very important. A setting resulting in a cut-off length of approximately thirty times the diameter of the claw tip proved most appropriate for describing the roughness order that is relevant for the functioning of the claws of E. assimilis larvae. Common technical roughness parameters such as R-a, R-q or R-z supplied sufficient differentiation between the tested substrata. E. assimilis larvae require a minimum surface roughness (R-a similar to 6.0 mu m) for their claws to grip. At lesser surface roughness, the larvae left the substratum. This was surprising because E. assimilis larvae have been observed to attach even to smooth surfaces. Based on the use of sterile substrata in this experiment, we assume that coverage with biofilm considerably influences the attachment of the larvae. (C) 2012 Elsevier GmbH. All rights reserved.