Evidence for stem cells in cultures of mouse prostate epithelial cells.
Evidence for stem cells in cultures of mouse prostate epithelial cells.
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小鼠前列腺上皮细胞培养物中存在干细胞的证据。
DOI：
10.1002/pros.10031
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Rothman,CraigJ
中科院分区：
文献类型：
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作者：
Sawicki,JanetA;Rothman,CraigJ
方法：对小鼠前列腺上皮细胞进行原代培养，以鉴定具有干细胞特征的细胞。免疫染色鉴定了表达细胞角蛋白5（K5）、细胞角蛋白8（K8）和bcl-2的细胞。通过重新接种原代集落中的解离细胞来鉴定具有克隆形成能力的细胞。结果仅2-4%的前列腺上皮细胞接种后形成集落。培养2-4天的大多数细胞表达bcl-2、K5和K8。培养6天后，集落具有两种不同的表型，星星形或盘形。在两种表型的集落中，只有少数细胞表达K5和bcl-2，而所有细胞都表达K8。重新铺板后，三分之二的星星形集落产生了一个单一的集落; 29盘形集落，没有产生新的colonies. CONCLUSIONSSColonies来自推定的前列腺上皮干细胞的鉴定。这些细胞表达K5和bcl-2，具有克隆形成性。前列腺50：46-53，2002年。© 2002 Wiley利斯公司
BACKGROUNDPrimary cultures of mouse prostatic epithelial cells were studied to identify cells having stem cell characteristics.METHODSCulture conditions supporting the growth of mouse prostatic epithelial cells were identified. Immunostaining identified cells expressing cytokeratin 5 (K5), cytokeratin 8 (K8), and bcl‐2. Cells with clonogenic capacity were identified by replating dissociated cells from primary colonies.RESULTSOnly 2–4% of prostatic epithelial cells that were plated formed colonies. Most cells cultured for 2–4 days expressed bcl‐2, K5, and K8. After 6 days in culture, colonies had two distinct phenotypes, star‐ or disc‐shaped. In colonies of both phenotypes, only a few cells expressed K5 and bcl‐2, while all cells expressed K8. Upon replating, two‐thirds of star‐shaped colonies gave rise to a single colony; of 29 disc‐shaped colonies, none gave rise to new colonies.CONCLUSIONSColonies deriving from putative prostatic epithelial stem cells are identified. These cells express K5 and bcl‐2 and are clonogenic. Prostate 50: 46–53, 2002. © 2002 Wiley‐Liss, Inc.